Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43464
Matise MP, et al., Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci. 1997 Oct 15;17(20):7805-16
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5467552
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Gene symbol:
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Pax2
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Gene name:
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paired box 2
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Antibody:
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Anti-Pax2
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
6a & 6b:
embryonic day 11.5
(more  )
Note: Double labeled: red - Pax2; green - Tubb3 (assay MGI:5467547)
Specimen
6a & 6b:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax2; green - Tubb3 (assay MGI:5467547)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
6a
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6b
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Expression was detected in differentiating neurons at the lateral margins of the ventricular zone in two domains; dorsal to the ventral horn in the ventral spinal cord and extending up to the sulcus limitans, and in the developing dorsal horn.
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Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future spinal cord |
Present |
Regionally restricted |
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Expression was detected in the intermediate region of the spinal cord.
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Specimen
7b & 7c:
embryonic day 9.5
(more )
Note: Double labeled: red - Pax2; green - Lhx1 (assay MGI:5467550) and Lhx5 (assay MGI:5467551)
Specimen
7b & 7c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax2; green - Lhx1 (assay MGI:5467550) and Lhx5 (assay MGI:5467551)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future spinal cord |
Present |
Regionally restricted |
7c
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7b
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In midthoracic spinal cord, expression in the ventral intermediate region overlaps with Lim1/2.
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Specimen
7e & 7f:
embryonic day 9.5
(more )
Note: Double labeled: red - Pax2; green - Lhx1 (assay MGI:5467550) and Lhx5 (assay MGI:5467551)
Specimen
7e & 7f:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax2; green - Lhx1 (assay MGI:5467550) and Lhx5 (assay MGI:5467551)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future spinal cord |
Present |
Regionally restricted |
7e
,
7f
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Expression was detected in dorsal and ventral domains and overlaps with Lim1/2. Staining in the ventral intermediate region is ventral to the sulcus limitans.
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Specimen
not shown:
embryonic day 11.5
(more )
Note: Also stained for Lhx1 (assay MGI:5467550) and Lhx5 (assay MGI:5467551)
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Also stained for Lhx1 (assay MGI:5467550) and Lhx5 (assay MGI:5467551)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
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Expression in the ventral horn showed less overlap with Lim1/2 at E11.5. However, many cells in the intermediate region continue co-expression. There is only partial overlap of expression with Lim1/2 in the dorsal horn.
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