Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43464
Matise MP, et al., Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci. 1997 Oct 15;17(20):7805-16
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5467409
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1a:
embryonic day 10.5; En1tm2Alj/En1 +
(more  )
Note: Double labeled: blue - En1 reporter; blue - Evx1 (assay MGI:5467299)
Specimen
1a:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: blue - En1 reporter; blue - Evx1 (assay MGI:5467299)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
1a
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Expression was detected in the ventral intermediate zone. Expression did not overlap with Evx1 expressing cells in an adjacent population of cells.
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Specimen
not shown:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: Also stained for Evx1 (assay MGI:5467299)
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Also stained for Evx1 (assay MGI:5467299)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
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The relationship of En1 to Evx1 expression was also seen in the hindbrain.
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| TS17: rhombomere 1 |
Absent |
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| TS17: rhombomere 2 |
Present |
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The rostral boundary of expression was at the border of rhombomeres 1 and 2.
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Specimen
not shown:
embryonic day 11.5; En1tm2Alj/En1 +
(more )
Note: Also stained for Evx1 (assay MGI:5467299)
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Also stained for Evx1 (assay MGI:5467299)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
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The relationship of En1 to Evx1 expression was also seen at E11.5.
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| TS19: hindbrain |
Present |
Regionally restricted |
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The relationship of En1 to Evx1 expression was also seen at E11.5.
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Specimen
1b:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: Double labeled: blue - En1 reporter; blue - Lmx1b (assay MGI:5467415)
Specimen
1b:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Agarose
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Note:
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Double labeled: blue - En1 reporter; blue - Lmx1b (assay MGI:5467415)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
1b
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Expression was detected in the ventral intermediate zone. Expression did not overlap with Lmx1b expressing cells.
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Specimen
1d:
embryonic day 11.5; En1tm2Alj/En1 +
(more )
Note: Double labeled: blue - En1 reporter; blue - Jag1 (assay MGI:5467510)
Specimen
1d:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Double labeled: blue - En1 reporter; blue - Jag1 (assay MGI:5467510)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Regionally restricted |
1d
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The region of the neuroepithelium from which the expressing cells derive corresponds to the region of Jag1 expression in the ventricular zone.
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Specimen
not shown:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: Also stained for Jag1 (assay MGI:5467510)
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Also stained for Jag1 (assay MGI:5467510)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
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The region of the neuroepithelium from which the expressing cells derive corresponds to the region of Jag1 expression in the ventricular zone.
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| TS17: hindbrain |
Present |
Regionally restricted |
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The region of the neuroepithelium from which the expressing cells derive corresponds to the region of Jag1 expression in the ventricular zone.
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Specimen
not shown:
embryonic day 11.5; En1tm2Alj/En1 +
(more )
Note: Also stained for Jag1 (assay MGI:5467510)
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Also stained for Jag1 (assay MGI:5467510)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
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The region of the neuroepithelium from which the expressing cells derive corresponds to the region of Jag1 expression in the ventricular zone.
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Specimen
1f:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: Double labeled: blue - En1 reporter; blue - Dll1 (assay MGI:5467512)
Specimen
1f:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Double labeled: blue - En1 reporter; blue - Dll1 (assay MGI:5467512)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
1f
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Expression was detected in the intermediate region corresponding to the gap in Dll1 expression in the ventricular zone.
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Specimen
1g:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: Double labeled: blue - En1 reporter; blue - Dbx1 (assay MGI:5467519)
Specimen
1g:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
|
En1tm2Alj/En1+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
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Not Specified
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|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Double labeled: blue - En1 reporter; blue - Dbx1 (assay MGI:5467519)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
1g
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Expression was detected in the intermediate region.
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Specimen
not shown:
embryonic day 9.5; En1tm2Alj/En1 +
(more )
Note: Also stained for Dbx1 (assay MGI:5467519)
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Agarose
|
|
Note:
|
Also stained for Dbx1 (assay MGI:5467519)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future spinal cord |
Present |
Regionally restricted |
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Expression was detected in the intermediate region.
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| TS15: central nervous system |
Present |
Regionally restricted |
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Expression was detected in the rostral neural tube from the midthoracic region and higher.
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Specimen
not shown:
embryonic day 11.5; En1tm2Alj/En1 +
(more )
Note: Also stained for Dbx1 (assay MGI:5467519)
Specimen
not shown:
(close )
|
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Also stained for Dbx1 (assay MGI:5467519)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
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Expression was detected in the intermediate region.
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Specimen
1h:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: Double labeled: blue - En1 reporter; blue - Pax2 (assay MGI:5467520)
Specimen
1h:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
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Double labeled: blue - En1 reporter; blue - Pax2 (assay MGI:5467520)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
1h
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Expression was detected in the intermediate region. The domain overlaps the ventral limit of Pax2 expression.
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Specimen
2b & 2c:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: This section was from the midlumbar region.
Specimen
2b & 2c:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This section was from the midlumbar region.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
2b
,
2c
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Expression was detected in postmitotic interneurons migrating out of the ventricular zone.
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Specimen
6c:
embryonic day 11.5; En1tm2Alj/En1 +
(more )
Note: Double labeled: blue - En1 reporter; brown - Pax2 (assay MGI:5467560)
Specimen
6c:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - En1 reporter; brown - Pax2 (assay MGI:5467560)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
6c
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Expression was detected in the ventral intermediate zone. Expression overlaps with Pax2.
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Specimen
8a:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Double labeled: blue - En1 reporter; blue - Evx1 (assay MGI:5467299)
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord |
Present |
Regionally restricted |
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Expression was detected in interneurons.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: spinal cord |
Present |
Regionally restricted |
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Expression was detected in interneurons.
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