Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43464
Matise MP, et al., Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci. 1997 Oct 15;17(20):7805-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5467519
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Gene symbol:
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Dbx1
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Gene name:
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developing brain homeobox 1
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Probe:
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c164
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Probe preparation:
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Antisense RNA
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Results
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Specimen
1g:
embryonic day 10.5; En1tm2Alj/En1 +
(more  )
Note: Double labeled: purple - Dbx1; blue - En1 reporter (assay MGI:5467409)
Specimen
1g:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: purple - Dbx1; blue - En1 reporter (assay MGI:5467409)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
1g
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Expression spans the ventricular and intermediate zones. The domain is immediately dorsal to the En1 expression domain.
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Specimen
not shown:
embryonic day 9.5; En1tm2Alj/En1 +
(more )
Note: Also stained for En1 reporter (assay MGI:5467409)
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Also stained for En1 reporter (assay MGI:5467409)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future spinal cord |
Present |
Regionally restricted |
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Expression spans the ventricular and intermediate zones. The domain is immediately dorsal to the En1 expression domain.
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| TS15: central nervous system |
Present |
Widespread |
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Expression was seen along the entire length of the neural tube.
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Specimen
not shown:
embryonic day 11.5; En1tm2Alj/En1 +
(more )
Note: Also stained for En1 reporter (assay MGI:5467409)
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Also stained for En1 reporter (assay MGI:5467409)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
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Expression spans the ventricular and intermediate zones. The domain is immediately dorsal to the En1 expression domain.
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