Antibody:	anti-axonin-1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	3A	Expression is in fibers run along the lateral and ventral surface of the midbrain toward the pons.
TS17: midbrain	Present	Regionally restricted	3A	Expression is in fibers run along the lateral and ventral surface of the midbrain toward the pons.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	3B	Expression is in fibers and small fiber bundles.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present	Regionally restricted	3C	Expression is in the oculomotor nerve.
TS17: hindbrain	Present	Regionally restricted	3C	Expression is in fibers run along the lateral and ventral surface of the midbrain toward the pons.
TS17: midbrain	Present	Regionally restricted	3C	Expression is in fibers run along the lateral and ventral surface of the midbrain toward the pons.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	3D	There is cell and fiber staining in the premigratory zone, there are stained processes extending back into the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS19: trigeminal ganglion	Weak	Regionally restricted	4A
TS19: cranial nerve	Very strong	Regionally restricted	4A	Expression is in the trigeminal motor root.
TS19: metencephalon basal plate	Very strong	Regionally restricted	4A	Expression is in fibers that compose a sheet that extends toward the trigeminal boundary cap.

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present	Regionally restricted	4B	In the lumbar region.

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Very strong	Regionally restricted	4C	Expression is in motor axons projecting through the ventral roots towards the mesenchyme of the forelimbs.
TS19: dorsal root ganglion	Present		4C
TS19: neural tube lateral wall	Present		4C	Expression is in circumferential fibres that run along the border between the ventricular and intermediate zones toward the motor column.

Structure	Level	Pattern	Image	Note
TS19: cerebellum primordium	Present	Regionally restricted	4D	Expression is in cell somata and short processes at the surface of the cerebellar neuroepithelium.

Structure	Level	Pattern	Image	Note
TS19: accessory nerve spinal component	Strong		4E

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Very strong		5A
TS20: spinal cord floor plate	Present		5A

Structure	Level	Pattern	Image	Note
TS20: spinal nerve	Present	Regionally restricted	5B

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		5C
TS20: cranial nerve	Present	Regionally restricted	5C	Olfactory nerve.

Structure	Level	Pattern	Image	Note
TS20: oculomotor nerve	Present	Regionally restricted	5D
TS20: midbrain	Present	Regionally restricted	5D	Expression is in neurons in the early forming lateral mammillary nucleus and their projecting axons toward the midbrain tegmentum.

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Moderate		5E	There is cellular and neuropil staining.
TS20: cranial nerve	Strong	Regionally restricted	5E	Expression is in the ophthalmic, maxillary and mandibular nerves including their projections into the mesenchyme of the respective target regions.
TS20: trigeminal nerve	Strong		5E

Structure	Level	Pattern	Image	Note
TS20: cranial nerve	Present		5F	Expression is in the abducens nerve.

Structure	Level	Pattern	Image	Note
TS20: glossopharyngeal nerve	Present		5G
TS20: hypoglossal nerve	Present		5G
TS20: medulla oblongata	Present		5G
TS20: trigeminal nerve	Present		5G
TS20: cranial nerve	Strong	Regionally restricted	5G	Expression is in the facial motor root.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present	Regionally restricted	5H	Ambiguus nucleus.
TS20: vagal nerve trunk	Very strong		5H
TS20: glossopharyngeal nerve	Strong	Regionally restricted	5H	Expression is in the jugular ganglion as well as its peripheral and central processes.
TS20: hypoglossal nerve	Strong		5H

Structure	Level	Pattern	Image	Note
TS20: optic stalk	Present		6A
TS20: hypothalamus	Present	Regionally restricted	6A	Exression is in cells and fibres of the lateral hypothalamic area.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Very strong	Regionally restricted	6B	Expression is in tangentially oriented fibres, many of which project into the midbrain tegmentum.
TS20: cerebellum primordium	Present		6B
TS20: cranial nerve	Present	Regionally restricted	6B	Expression is in the trochlear nerve.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Moderate	Regionally restricted	6C	Expression is in processes that extend into the primordium.
TS20: medulla oblongata mantle layer	Strong	Regionally restricted	6C	Expression is in chains composed of fibers and spindle-shaped cells that extend ventrally toward the marginal and mantle zone of the medulla.
TS20: medulla oblongata marginal layer	Strong	Regionally restricted	6C	Expression is in chains composed of fibers and spindle-shaped cells that extend ventrally toward the marginal and mantle zone of the medulla.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		6D

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	6E	Expression is in the most superficial layer of the rostrobasal wall of the telencephalic vesicle, where the olfactory bulb will form and in the primordial plexiform layer of lateral aspects of the telencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Strong		7A
TS21: spinal cord lateral wall	Present		7A	Expression is in dorsal funiculi, absent in ventral horn. Expression is in commissural axons in a medial trajectory along the border of the ventricular zone toward the floor plate.
TS21: spinal cord floor plate	Present		7A
TS21: basal columns	Absent		7A

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Present	Regionally restricted	7B

Structure	Level	Pattern	Image	Note
TS21: spinal nerve	Very strong		7C

Structure	Level	Pattern	Image	Note
TS21: spinal nerve	Very strong		7D

Structure	Level	Pattern	Image	Note
TS21: spinal nerve	Present	Regionally restricted	7E	Expression is in fibers in the thoracic rami communicantes as well as coursing along the thoracic sympathetic chain. Some extend into the cervical and lumbar sympathetic chain, but most of them enter the developing splanchnic nerves.
TS21: sympathetic ganglion	Absent		7E

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Present		7F
TS21: olfactory lobe	Very strong	Regionally restricted	7F	Expression is in cells and fibers in the intermediate zone of its medial aspect. Fibers extend laterally from this zone toward the basolateral surface of the telencephalic vesicle.
TS21: olfactory nerve	Very strong		7F

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Present	Single cells	7G	Sensory cells.
TS21: olfactory nerve	Very strong		7G

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Very strong	Regionally restricted	7H	Staining is present in central and peripheral processes of the ganglion, while the motor root of the mandibular nerve is unstained.
TS21: trigeminal nerve mandibular division	Present	Regionally restricted	7H	Expression is in sensory fibres.
TS21: trigeminal nerve maxillary division	Present	Regionally restricted	7H	Expression is in sensory fibres.
TS21: trigeminal nerve ophthalmic division	Present	Regionally restricted	7H	Expression is in sensory fibres.
TS21: cranial nerve	Present	Regionally restricted	7H	Absent in trochlear and abducens nerves as well as the trigeminal motor root.
TS21: oculomotor nerve	Absent		7H

Structure	Level	Pattern	Image	Note
TS21: facial nerve	Very strong		7I
TS21: vestibular ganglion	Moderate		7I	In the peripheral processes of the vestibular ganglion.
TS21: cochlear ganglion	Moderate		7I
TS21: inferior vagus ganglion	Present	Regionally restricted	7I	Nodose ganglion.

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Present	Regionally restricted	7J	Expression is in the solitary tract.

Structure	Level	Pattern	Image	Note
TS21: superior vagus ganglion	Present	Regionally restricted	7K	Expression is in the jugular ganglion, from which a bundle of very strongly immunoreactive central fibers project to the medulla and join the spinal trigeminal tract.
TS21: medulla oblongata	Present	Regionally restricted	7K	Expression is in the solitary tract.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex intermediate zone	Very strong		8A	Expression is in cells and fibres of the ventrolateral cortical intermediate zone.
TS21: forebrain	Present	Regionally restricted	8A
TS21: trigeminal nerve mandibular division	Present	Regionally restricted	8A	Expression is in sensory fibres.
TS21: trigeminal nerve maxillary division	Present	Regionally restricted	8A	Expression is in sensory fibres.
TS21: trigeminal nerve ophthalmic division	Present	Regionally restricted	8A	Expression is in sensory fibres.
TS21: telencephalon	Present	Regionally restricted	8A	Expression is in fibers that project toward the ganglionic eminence, but not past the eminence. The forming internal capsule remains mostly devoid of staining.
TS21: cranial nerve	Present	Regionally restricted	8A	Absent in trochlear and abducens nerves as well as the trigeminal motor root.
TS21: olfactory cortex	Absent		8A	Absent in piriform cortex and in septal regions.
TS21: oculomotor nerve	Absent		8A

Structure	Level	Pattern	Image	Note
TS21: thalamus	Strong	Regionally restricted	8B	Expression is in fibers of the mammillothalamic tracts over the surface of the developing anterior thalamic nuclei. Weak in the zone of the ventrolateral thalamic migratory stream.
TS21: forebrain	Present	Regionally restricted	8B	Weak in the mammillary body, but the mammillotegmental and mammillothalamic tracts are very intensely stained and fibers of the latter can be seen to distribute over the surface of the developing anterior thalamic nuclei.
TS21: telencephalon	Present	Regionally restricted	8B	Expression is underneath the basal telencephalic neuroepithelium in the intermediate zone of the amygdala.

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present	Regionally restricted	8C	Expression is in fibers of the mammillothalamic tracts over the surface of the developing anterior thalamic nuclei and in spindle-shaped cells and in their horizontally oriented processes. Moderate in fibers of the stria medullaris.
TS21: midbrain	Present	Regionally restricted	8C	Expression is in fibers that approach the thalamus from the midbrain.
TS21: epithalamus	Present	Regionally restricted	8C	In cells that concentrate in the region of the medial habenula, from where the very strongly stained habenulointerpeduncular tract descends toward the mesencephalic flexure.
TS21: brainstem	Strong	Regionally restricted	8C	Expression is in the region of the posterior commissure.

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Present	Regionally restricted	8D	Expression is in the rostral part of the lateral hypothalamic area.
TS21: forebrain	Present	Regionally restricted	8D	Expression is in a medial forebrain bundle.
TS21: neural retinal epithelium	Very strong	Regionally restricted	8D	Expression is in axons in the central retina.
TS21: optic stalk	Present		8D

Structure	Level	Pattern	Image	Note
TS21: optic stalk	Present		8E

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	8F	Expression is in fibers on the lateral surface of the tegmentum.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	8G	Expression is in fibers and small group of cells that cross the midline tegmentum and dense fiber staining along the lateral and ventral surface.
TS21: brainstem	Present	Regionally restricted	8G	Expression is in the lower brainstem.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present	Regionally restricted	8H	Expression is in two distinct layers. Weak in the region of the inferior cerebellar peduncle and stronger in a deeper layer in the cerebellar primordium which are rows of vertically oriented cells.

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Present	Regionally restricted	8I	Staining is over the fasciculus uncinatus which runs in a transverse direction and crosses the midline over the surface of the postisthmal part of the cerebellar anlage.
TS21: midbrain	Present	Regionally restricted	8I	Expression is in fibers that cross the midline tegmentum.
TS21: pons	Present	Regionally restricted	8I	Expression is near the pial surface.

Structure	Level	Pattern	Image	Note
TS22: cochlear ganglion	Present
TS22: diencephalon	Present
TS22: glossopharyngeal ganglion	Present
TS22: vagus ganglion	Present
TS22: vestibular ganglion	Absent

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	9A	Expression is in spinal commissural neurons.

Structure	Level	Pattern	Image	Note
TS22: trigeminal nerve maxillary division	Very strong	Regionally restricted	9B	Expression is in axons of the infraorbital branch of the maxillary nerve that contact the developing follicles of the whisker pad.
TS22: facial nerve	Present	Regionally restricted	9B	Absent in the nucleus, present in the facial motor root.

Structure	Level	Pattern	Image	Note
TS22: retina	Very strong	Regionally restricted	9C	Expression is in ganglion cells underneath the very intensely stained nerve fibre layer into which they send their axons. Absent in t he outer layers of the retina.
TS22: optic chiasma	Very strong		9C
TS22: optic nerve	Very strong		9C

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	9D	Expression is weak except for a strong subpial band which extends around the surface of the medulla, the left and right components being continuous across the midline.
TS22: hindbrain	Present	Regionally restricted	9D	Expression is in precerebellar premigratory zones.
TS22: facial nerve	Present	Regionally restricted	9D	Motor nucleus.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Regionally restricted	9E	Cell associated expression has mostly disappeared. Expression is in precerebellar premigratory zones.
TS22: facial nerve	Present		9E	Motor nucleus.
TS22: midbrain	Present	Regionally restricted	9E	Expression is in longitudinal fibers that extend from the midbrain tegmentum into the pons.
TS22: pons	Present	Regionally restricted	9E	Expression is in longitudinal fibers that extend from the midbrain tegmentum into the pons.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	9F	Expression is weak except for a strong subpial band which extends around the surface of the medulla, the left and right components being continuous across the midline.
TS22: medulla oblongata lateral wall	Present	Scattered	9F	Expression is in transverse fibers in the mantle zone of the medulla.
TS22: hindbrain	Present	Regionally restricted	9F	Cell associated expression has mostly disappeared.
TS22: facial nerve	Present	Regionally restricted	9F	Motor nucleus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex mantle layer	Present	Regionally restricted	9G	In the lateral cortical mantle in its superficial zone.
TS22: cerebral cortex intermediate zone	Strong	Regionally restricted	9G	Expression is in fibers.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Very strong	Regionally restricted	10A	Expression is in the lateral aspect of the dorsal funiculi, weak in commissural and associative fibre systems, high in association fibres of the lumbar spinal cord.
TS23: dorsal root ganglion	Present		10A
TS23: spinal nerve plexus	Present	Regionally restricted	10A	In smaller branches of the spinal nerves.

Structure	Level	Pattern	Image	Note
TS23: central nervous system nerve	Present	Regionally restricted	10B	Expression is in vagal trunks along the oesophagus, absent in the region of the cardia.

Structure	Level	Pattern	Image	Note
TS23: trigeminal ganglion	Weak		10C
TS23: facial nerve	Very strong		10C

Structure	Level	Pattern	Image	Note
TS23: brainstem	Very strong	Regionally restricted	10D	In the zone of the lateral lemniscus.
TS23: pons	Present		10D
TS23: hindbrain	Present	Regionally restricted	10D	Expression is in transverse fibers, strong in in tectobulbar projections and in predorsal bundle.
TS23: midbrain tegmentum	Absent		10D

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Present	Regionally restricted	10E	Expression is in the fasciculus uncinatus, weak to moderate along the inner rim,in the premigratory zone next to the precerebellar neuroepithelium.
TS23: hindbrain	Present	Regionally restricted	10E	Expression is in transverse fibers.

Structure	Level	Pattern	Image	Note
TS23: cranial nerve	Very strong	Regionally restricted	10F	Expression is in the auricular branch of the vagus nerve including its cutaneous ramifications. Single strongly stained fibers are also found in the proximal stem of the vagus nerve and in branches that project toward the pharynx and cervical viscera.

Structure	Level	Pattern	Image	Note
TS23: vestibulo-cochlear ganglion	Weak
TS23: vestibulocochlear nerve	Present	Regionally restricted		Expression is in peripheral and central processes.
TS23: hypoglossal nerve	Present	Regionally restricted		In some of the rootlets.

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Present	Regionally restricted	11A	Expression is in the rostral branch of the anterior commissure.
TS23: olfactory cortex	Present	Regionally restricted	11A	Lateral olfactory tract.
TS23: cerebral cortex mantle layer	Present		11A
TS23: forebrain	Present		11A	Expression is in the intermediate zone of the developing Ammon's horn. From its rostral aspects, a bundle of moderately stained fornical fibers extend to the medial surface of the septa1 region.
TS23: hippocampus	Present		11A	Expression is in the intermediate zone of the developing Ammon's horn. From its rostral aspects, a bundle of moderately stained fornical fibers extend to the medial surface of the septa1 region.

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Present	Regionally restricted	11B	Expression is in the superior cerebellar peduncle.
TS23: superior colliculus	Present	Regionally restricted	11B	Expression is in a band lining the ventricular zone.
TS23: midbrain tegmentum	Absent		11B

Structure	Level	Pattern	Image	Note
TS23: thalamus	Strong	Regionally restricted	11C	Expression is in cells and processes underneath the neuroepithelium of the anteromedial nucleus, virtually absent from other nuclei of the thalamus. Strongly in fibers that invade the posterior thalamus.
TS23: epithalamus	Strong	Regionally restricted	11C	Expression is in habenula.
TS23: brainstem	Strong	Regionally restricted	11C	Expression is in the region of the posterior commissure.
TS23: midbrain	Strong	Regionally restricted	11C	Expression is in fascicles of the habenulointerpeduncular tract.

Structure	Level	Pattern	Image	Note
TS23: hypothalamus	Present	Regionally restricted	11D	Expression is in the rostral part of the anterior hypothalamic area.
TS23: forebrain	Strong	Regionally restricted	11D	Expression is in the medial forebrain bundle.
TS23: neural retina	Strong	Regionally restricted	11D	Retinal ganglion cells.
TS23: optic chiasma	Very strong	Regionally restricted	11D
TS23: optic nerve	Very strong	Regionally restricted	11D

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Present	Regionally restricted	11E	Weak in mammillary body and mamillothalamic tract, but strong in the mammillotegmental tract.
TS23: thalamus	Moderate	Regionally restricted	11E	Expression is in the stria medullaris.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Very strong		12A
TS24: cortical plate	Very strong		12A

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Very strong		12B
TS24: hippocampus	Very strong		12B
TS24: diencephalon	Very strong	Regionally restricted	12B	Expression is in the medial habenula next to the venrticular zone. Their axons join the very intensely stained habenulointerpeduncular tract. Present in the rostral branches of the anterior commissure, weak at the midline.
TS24: thalamus	Very strong	Regionally restricted	12B	Expression is in the medial habenula next to the venrticular zone. Their axons join the very intensely stained habenulointerpeduncular tract. Present in the rostral branches of the anterior commissure, weak at the midline.
TS24: forebrain	Present	Regionally restricted	12B	Expression is in some fibres of the medial forebrain bundle, the mammillotegmental tract, the stria medullaris, and the anteromedial thalamic nucleus.

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Very strong		12C
TS24: hippocampus	Very strong		12C
TS24: brainstem	Present	Regionally restricted	12C	Expression is in fibers in the posterior commissure.
TS24: midbrain	Present	Regionally restricted	12C	Staining is in fibers that form the cerebral peduncle.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Moderate		13A
TS24: lumbosacral plexus	Moderate		13A

Structure	Level	Pattern	Image	Note
TS24: spinal component of peripheral nervous system	Present	Regionally restricted	13B	Expression is in sacral nerves give off fibers that form a plexus around the rectum.

Structure	Level	Pattern	Image	Note
TS24: spinal component of peripheral nervous system	Moderate	Regionally restricted	13C	Expression is in a plexus along the abdominal aorta and along the mesenteric, coeliac and renal arteries.

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present		13D
TS24: olfactory nerve	Weak		13D
TS24: olfactory epithelium	Absent		13D

Structure	Level	Pattern	Image	Note
TS24: pons	Present	Regionally restricted	13E	Expression is in the pontine migration and is almost absent at the zone of the forming pontine nuclei. Moderate staining is still seen in medial and lateral tectopontine tracts, but the predorsal bundle can no longer be traced past the pons.

Structure	Level	Pattern	Image	Note
TS24: pons	Present	Regionally restricted	13F	Expression is in the pontine migration.
TS24: medulla oblongata	Present	Regionally restricted	13F	Expression is in the spinal trigeminal tract, absent in transverse and commissural fibers.

Structure	Level	Pattern	Image	Note
TS24: inferior colliculus	Weak
TS24: hypothalamus	Trace	Regionally restricted		Parctically disappeared from the hypothalamus, thalamus, and tectothalamic efferents.
TS24: thalamus	Trace	Regionally restricted		Parctically disappeared from the hypothalamus, thalamus, and tectothalamic efferents.
TS24: superior colliculus	Absent

Structure	Level	Pattern	Image	Note
TS25: hippocampus	Very strong
TS25: inferior colliculus	Weak
TS25: superior colliculus	Absent

Structure	Level	Pattern	Image	Note
TS25: retina nerve fiber layer	Strong		14A
TS25: neural retina	Present	Single cells	14A	Expression is in ganglion cells in the peripheral retina.

Structure	Level	Pattern	Image	Note
TS25: central nervous system nerve	Present	Regionally restricted	14B	Peripheral cutaneous branches.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Very strong	Regionally restricted	15A	Expression is in the premigratory zone along its inner rim.
TS27: pons	Very strong	Regionally restricted	15A	Pontine migration.
TS27: dentate gyrus hilus	Moderate		15A
TS27: dentate gyrus molecular layer	Moderate		15A
TS27: hippocampus stratum lacunosum	Moderate		15A
TS27: cerebral cortex	Very strong	Regionally restricted	15A	Cortical plate and moderately stained axons which, coming from motor cortical regions, course through the internal capsule into the cerebral peduncle.
TS27: fimbria hippocampus	Very strong		15A
TS27: hippocampus pyramidal cell layer	Very strong		15A
TS27: olfactory tract	Very strong		15A
TS27: hippocampus	Very strong	Regionally restricted	15A	Alveus hippocampus.
TS27: thalamus	Strong	Regionally restricted	15A	Expression is in two sets of fibers.
TS27: anterior commissure	Strong	Regionally restricted	15A	Anterior branch, weak in posterior branch.
TS27: olfactory nerve	Absent		15A
TS27: subiculum	Absent		15A

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong	Regionally restricted	15B	Expression is in the premigratory zone along its inner rim.
TS27: pons	Very strong	Regionally restricted	15B	Pontine migration.
TS27: hippocampus	Very strong	Regionally restricted	15B	Alveus hippocampus.
TS27: fimbria hippocampus	Very strong		15B
TS27: hippocampus pyramidal cell layer	Very strong		15B
TS27: olfactory tract	Very strong		15B
TS27: optic nerve	Very strong		15B
TS27: optic tract	Very strong		15B
TS27: anterior commissure	Strong	Regionally restricted	15B	Anterior branch, weak in posterior branch.
TS27: cerebral cortex	Present	Regionally restricted	15B	Expression in fibers, superficial cells and in tracts, very strong in the cortical plate.
TS27: dentate gyrus hilus	Moderate		15B
TS27: dentate gyrus molecular layer	Moderate		15B
TS27: hippocampus stratum lacunosum	Moderate		15B
TS27: olfactory nerve	Absent		15B
TS27: retina ganglion cell layer	Absent		15B
TS27: subiculum	Absent		15B

Structure	Level	Pattern	Image	Note
TS27: olfactory tract	Strong		16A
TS27: anterior commissure	Strong		16A	Anterior branch, weak in posterior branch.
TS27: corpus callosum	Very strong		16A
TS27: olfactory nerve	Absent		16A

Structure	Level	Pattern	Image	Note
TS27: habenula	Very strong	Regionally restricted	16B	In the habenulointerpeduncular tract and the most medial portion of the habenula.
TS27: thalamus	Very strong	Regionally restricted	16B	In corticothalamic fibres that extend from the internal capsule into the ventroposterior nucleus of the thalamus. The fibres gradually loose staining as they penetrate the nuclei and no growth cones can be identified.
TS27: hippocampus	Very strong	Regionally restricted	16B	Alveus hippocampus.
TS27: olfactory tract	Strong		16B
TS27: cerebral cortex	Strong	Regionally restricted	16B	In corticofugal fibres that arise from a slightly more lateral cortical zone.
TS27: fimbria hippocampus	Present		16B
TS27: olfactory nerve	Absent		16B

Structure	Level	Pattern	Image	Note
TS27: habenula	Very strong	Regionally restricted	16C	In the habenulointerpeduncular tract and the most medial portion of the habenula.
TS27: hippocampus	Very strong	Regionally restricted	16C	Alveus hippocampus.
TS27: fimbria hippocampus	Very strong		16C
TS27: thalamus	Present		16C	Expression is in mamillothalamic tract.
TS27: cerebral cortex	Present	Regionally restricted	16C	In corticofugal fibres that arise from a slightly more lateral cortical zone.In corticofugal fibres that arise from a slightly more lateral cortical zone.

Structure	Level	Pattern	Image	Note
TS27: pons	Present	Regionally restricted	17A	Staining is in pontine migration.
TS27: hippocampal commissure	Strong		17A
TS27: corpus callosum	Strong	Regionally restricted	17A	Expression is in the midplane itself and on axons as they course from the respective cortical areas to the midplane.
TS27: anterior commissure	Present		17A
TS27: fornix	Absent		17A

Structure	Level	Pattern	Image	Note
TS27: cerebral hemisphere white matter	Present		17B
TS27: inferior colliculus	Present		17B

Structure	Level	Pattern	Image	Note
TS27: pons	Present		17C	Pontine migration.

Structure	Level	Pattern	Image	Note
TS27: trigeminal nerve	Present		17D

Structure	Level	Pattern	Image	Note
TS27: pons	Present	Regionally restricted	17E	Pontine migration.

Structure	Level	Pattern	Image	Note
TS28: olfactory tract	Very strong		18A
TS28: anterior commissure	Present		18A
TS28: corpus callosum	Weak		18A

Structure	Level	Pattern	Image	Note
TS28: olfactory tract	Very strong	Regionally restricted	18B	Absent at caudal levels.
TS28: hippocampal commissure	Moderate	Regionally restricted	18B
TS28: corpus callosum	Weak		18B

Structure	Level	Pattern	Image	Note
TS28: brain white matter	Present		18C
TS28: cerebral hemisphere white matter	Present		18C
TS28: ventral posterior lateral thalamic nucleus	Present		18C
TS28: ventral posterior medial thalamic nucleus	Present		18C
TS28: superior colliculus	Present	Regionally restricted	18C	Brachium of the superior colliculus.
TS28: cerebral cortex	Present	Regionally restricted	18C	In part of the retrosplenial cortex.
TS28: CA1 pyramidal cell layer	Weak		18C
TS28: dentate gyrus hilus	Weak		18C
TS28: fimbria hippocampus	Weak		18C	Absent in the ventromedial area.

Structure	Level	Pattern	Image	Note
TS28: optic nerve	Absent		19A

Structure	Level	Pattern	Image	Note
TS28: optic nerve	Absent		19B

Structure	Level	Pattern	Image	Note
TS28: optic tract	Moderate	Regionally restricted	19C	Expression is in the optic tract over the lateral geniculate nucleus.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Moderate		19D

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Very strong	Regionally restricted	19E	Expression is in the premigratory zone.
TS28: cerebellum	Present	Single cells	19E	Expression is in some cells that are migrating towards the deep granular layer, absent in the subpial proliferative zone.

Structure	Level	Pattern	Image	Note
TS28: anterior commissure	Strong		19F
TS28: olfactory tract	Strong	Regionally restricted	19F	Expression is in fascicles of the beginning lateral olfactory tract.
TS28: main olfactory bulb internal plexiform layer	Moderate		19F

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Very strong	Regionally restricted	19G	Expression is in the premigratory zone.
TS28: cerebellum molecular layer	Very strong		19G

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Very strong	Regionally restricted	19H	Expression is in the outer third.