Summary | Transgene origin | Transgene description | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(Cd4-cre/ERT2)11Gnri
Name:	transgene insertion 11, Fotini Gounari
MGI ID:	MGI:5464279
Synonyms:	CD4CreERT2
Transgene:	Tg(Cd4-cre/ERT2)11Gnri  Location: unknown
Alliance:	Tg(Cd4-cre/ERT2)11Gnri page

Strain of Origin:

C57BL/6

Transgene Type:    Transgenic (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Tg(Cd4-cre/ERT2)11Gnri expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note Not Specified Cd4   Mutation details: A fragment encoding the cre recombinase fused to a mutated ligand-binding domain of the estrogen receptor (ERT2) was cloned downstream of the Cd4 gene promoter/enhancer/silencer to generate the transgene construct. The construct also contained the human growth hormone (hGH) polyadenylation sequence. Pronuclei of fertilized eggs were microinjected with the purified construct. Founder lines 11 and 19 were established. These were crossed to R26R-EYFP reporter mice. CD4 T cells were isolated from tamoxifen-treated double mutant animals and 50 and 30% of cells analyzed by FACS from lines 11 and 19 respectively showed EYFP expression. Line 11 had greater recombinase activity and was used for further analyses. (J:191897)

Generation of the Tg(Cd4-cre/ERT2)11Gnri transgene.

Activity:	Tissue activity of this recombinase allele
Driver:	Cd4 (species not specified) Summary of all recombinase alleles driven by Cd4.

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:191897 Aghajani K, et al., Generation of CD4CreER(T2) transgenic mice to study development of peripheral CD4-T-cells. Genesis. 2012 Dec;50(12):908-13
All:	39 reference(s)