Probe:

Raldh3 probe2

Structure	Level	Pattern	Image	Note
TS12: head	Absent		2a E8.0

Structure	Level	Pattern	Image	Note
TS12: head	Absent		2a E8.3

Structure	Level	Pattern	Image	Note
TS13: head	Present	Regionally restricted	2a E8.5	Expression was detected in the rostral head.
TS13: surface ectoderm	Present	Regionally restricted	2a E8.5	Expression was detected in the epidermis covering the posterior part of the optic recess and extending behind it.

Structure	Level	Pattern	Image	Note
TS13: head	Strong	Regionally restricted	2a E8.8	Expression became intense in the rostral head.
TS13: future spinal cord	Present	Regionally restricted	2a E8.8	Expression was seen in streaks along the spinal cord.
TS13: surface ectoderm	Strong	Regionally restricted	2a E8.8	Intense expression was detected in the surface ectoderm in a triangular-shaped area around the eye.

Structure	Level	Pattern	Image	Note
TS15: eye	Present		2b
TS15: lens placode	Present		2b
TS15: olfactory placode	Present		2b
TS15: head	Present	Regionally restricted	2b	Expression was seen in the eye/olfactory region and adjoining face.
TS15: surface ectoderm	Present	Regionally restricted	2b	Expression was seen in the eye/olfactory region and adjoining face.

Structure	Level	Pattern	Image	Note
TS16: eye	Present		2c
TS16: lens pit	Present		2c
TS16: nasal pit	Present		2c
TS16: head	Present	Regionally restricted	2c	Expression was seen in the eye/olfactory region and adjoining face. The labeled area is still contiguous, but is starting to constrict down to the two placodes.
TS16: surface ectoderm	Present	Regionally restricted	2c	Expression was seen in the eye/olfactory region and adjoining face. The labeled area is still contiguous, but is starting to constrict down to the two placodes.

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present		2d
TS17: nasal pit	Present		2d
TS17: otocyst	Present		2d

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Absent		2e	When the lens closed, expression was lost.
TS19: nasal process ectoderm	Absent		2e	As the olfactory pit develops into the olfactory organ, expression disappears from the external surface of the nose.
TS19: olfactory epithelium	Strong	Regionally restricted	2e	The internal epithelium of the olfactory organ was heavily stained.
TS19: eye	Present	Regionally restricted	2e	Surface labeling was in the eye region in a rectangular shape. It covered the eye obliquely, with a row of labeled cells extending into the naso-lacrimal groove.
TS19: naso-lacrimal groove	Present		2e
TS19: pigmented retinal epithelium	Present	Regionally restricted	2e	Expression was in the dorsal region.

Structure	Level	Pattern	Image	Note
TS22: retina	Strong	Regionally restricted	2f E14	Expression was detected in the ventral retina.

Structure	Level	Pattern	Image	Note
TS23: retina	Strong	Regionally restricted	2f E15	Expression was detected in the ventral retina.

Structure	Level	Pattern	Image	Note
TS27: retina	Ambiguous		2f P0.5	Expression in the retina slowly disappears postnatally.

Structure	Level	Pattern	Image	Note
TS14: eye	Strong		3_RALDH3
TS14: head	Strong	Regionally restricted	3_RALDH3	Expression was detected around the entire eye region and significantly beyond it.

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Present	Regionally restricted	4a	Expression was detected in the epidermis covering the posterior part of the optic recess and extending behind it.

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Strong	Regionally restricted	4b	Expression was detected in the surface ectoderm in a triangular-shaped area around the eye.

Structure	Level	Pattern	Image	Note
TS15: lens placode	Strong		4c
TS15: optic cup	Present	Regionally restricted	4c	Expression was detected in the dorsal retinal pigmented epithelium and the ventral retina.

Structure	Level	Pattern	Image	Note
TS16: nasal pit	Strong		4d

Structure	Level	Pattern	Image	Note
TS18: lens pit	Present		4e

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Absent		4f	When the lens closed, expression was lost.
TS20: retina	Present	Graded	4f	Expression covers the ventral two-thirds of the retina up to a horizontal border above the optic disc. The expression forms a ventro-dorsal gradient.

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	5a	Expression was detected in the ventral cortex.
TS20: lateral ganglionic eminence	Present	Regionally restricted	5a	Strongest staining was in the rostral part.
TS20: medial ganglionic eminence	Absent		5a

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Present		5b
TS21: telencephalon	Present	Regionally restricted	5b	Expression was in a streak extending from the piriform cortex with a long arm deep into the lateral ganglionic eminence and a short arm into the septum.
TS21: medial ganglionic eminence	Absent		5b

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Present	Regionally restricted	5c
TS22: piriform cortex	Present		5c
TS22: telencephalon septum	Present		5c
TS22: medial ganglionic eminence	Absent		5c

Structure	Level	Pattern	Image	Note
TS13: head	Present		6a RALDH3

Structure	Level	Pattern	Image	Note
TS14: surface ectoderm	Present		6b

Structure	Level	Pattern	Image	Note
TS19: head	Present	Regionally restricted	6e	Expression was detected in the eyelid region.
TS19: retina	Present	Regionally restricted	6e	Expression was detected in ventral and dorsal compartments.
TS19: nose	Present		6e
TS19: inner ear	Weak	Regionally restricted	6e	Expression was weak in the otic vesicle.