Probe:	Dlx2 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Two probes were described - MGI:5463648 and MGI:3767604. Either may have been used in this assay. Authors indicate that both probes give equivalent results.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	4A/B	Expression was detected in neuroepithelial zones in the outer germinal or synthetic layer.
TS19: telencephalon ventricular layer	Absent		4A/B	There was no expression in the proliferative zone directly adjacent to the ventricle.
TS19: diencephalon	Strong	Regionally restricted	4A/B	Expression was detected in the posterior hypothalamus.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	4C/D	Expression was detected in the neuroepithelia of the basal telencephalon.
TS19: diencephalon	Strong	Regionally restricted	4C/D	Expression was detected in the preoptic area.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong	Regionally restricted	5A/B	Expression was detected in the neuroepithelium of the basal telencephalon. No expression was found in the neuroepithelium of the cortex or hippocampus. Expression was strong in the synthetic zone and weaker in the differentiating zone.
TS21: cerebral cortex	Absent		5A/B

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong	Regionally restricted	5C/D	Expression was detected in the neuroepithelium of the basal telencephalon and in the diagonal band of Broca. Expression was strong in the synthetic zone and weaker in the differentiating zone.

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Present	Regionally restricted	5E/F	Expression was strong in the preoptic region. Weaker signal was detected in the anterior and lateral hypothalamus.
TS21: thalamus	Weak	Regionally restricted	5E/F	Weak expression was detected in the zona incerta.
TS21: diencephalon	Present	Regionally restricted	5E/F	The dorsal limit of expression in the diencephalon was at the junction of the basal and alar plates, the sulcus limitans.

Structure	Level	Pattern	Image	Note
TS24: olfactory lobe	Present	Regionally restricted	6A/B	Expression was detected in the ventrolateral neuroepithelium of the olfactory lobes.
TS24: caudate-putamen	Present		6A/B
TS24: telencephalon septum	Present		6A/B
TS24: main olfactory bulb	Present	Regionally restricted	6A/B	Expression was detected in the ventral region of the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS24: telencephalon septum	Present		6C/D
TS24: telencephalon	Strong	Regionally restricted	6C/D	Expression was detected in the basal telencephalon.
TS24: hypothalamus	Present	Regionally restricted	6C/D	Expression was detected in the medial preoptic area.

Structure	Level	Pattern	Image	Note
TS24: telencephalon septum	Strong	Regionally restricted	6E/F	Intense expression was detected in the synthetic and differentiating zones of the septal neuroepithelium.
TS24: cerebral cortex	Absent		6E/F
TS24: hippocampus	Absent		6E/F
TS24: caudate-putamen	Present		6E/F

Structure	Level	Pattern	Image	Note
TS24: telencephalon septum	Strong	Regionally restricted	6G/H	Intense expression was detected in the synthetic and differentiating zones of the septal neuroepithelium.
TS24: cerebral cortex	Absent		6G/H
TS24: hippocampus	Absent		6G/H
TS24: hypothalamus	Present	Regionally restricted	6G/H	Expression was detected in the medial preoptic area and the anterior hypothalamus. Expression was not evenly dispersed, but occurred in waves. No expression was found in the suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	6I/J	Expression was detected in the arcuate nucleus.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted		Expression was detected in the neuroepithelial zones of the diagonal band of Broca.
TS24: diencephalon	Weak	Regionally restricted		Weak expression was detected in the paraventricular nucleus.
TS24: zona incerta	Weak

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted		At E18.5, the location of expression was similar to that seen at E16.5. However, the intensity of the staining was decreased.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	7A/B	Expression was detected in the ventrolateral neuroepithelial cells of the synthetic zone. No expression was seen dorsally.

Structure	Level	Pattern	Image	Note
TS27: caudate-putamen	Moderate		7C/D

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Ambiguous			Expression was low or undetectable throughout the diencephalon, including the paraventricular and arcuate nucleus.

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Present

Structure	Level	Pattern	Image	Note
TS24: lower jaw incisor	Present	Regionally restricted	8A/B	Expression was detected in the ameloblasts.
TS24: upper jaw incisor	Present	Regionally restricted	8A/B	Expression was detected in the ameloblasts.

Structure	Level	Pattern	Image	Note
TS27: incisor	Present		8C/D
TS27: molar	Present		8C/D