Probe:	Pax9 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present	Regionally restricted		Expression is in the epithelium of the foregut.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial pouch	Strong		4A
TS15: 2nd branchial pouch	Strong		4A
TS15: 3rd branchial pouch	Strong		4A
TS15: hindgut	Strong		4A
TS15: sclerotome	Strong		4A
TS15: tail	Strong		4A
TS15: trunk mesenchyme	Strong		4A	Expression is in the mesenchyme underlying the hindgut.
TS15: tail unsegmented mesenchyme	Absent		4A

Structure	Level	Pattern	Image	Note
TS17: 1st branchial pouch	Strong		4C
TS17: 2nd branchial pouch	Strong		4C
TS17: 3rd branchial pouch	Strong		4C
TS17: 4th branchial pouch	Strong		4C
TS17: hindgut	Strong		4C
TS17: nasal process	Strong		4C
TS17: head	Strong	Regionally restricted	4C	Expression is at multiple sites withing the nonneural tissues.
TS17: trunk mesenchyme	Strong	Regionally restricted	4C	Expression is in the mesenchyme underlying the hindgut.
TS17: tail	Present		4C
TS17: sclerotome	Strong		4C	Along the entire body axis up to the caudal-most somite.

Structure	Level	Pattern	Image	Note
TS19: footplate	Strong	Regionally restricted	4E	Expression is in the anterior proximal corner.
TS19: handplate	Strong	Regionally restricted	4E	Expression is in the anterior proximal corner.
TS19: 1st branchial pouch	Strong		4E
TS19: 2nd branchial pouch	Strong		4E
TS19: 3rd branchial pouch	Strong		4E
TS19: 4th branchial pouch	Strong		4E
TS19: hindgut	Strong		4E
TS19: sclerotome	Strong		4E
TS19: head	Strong	Regionally restricted	4E	Expression is at multiple sites withing the nonneural tissues.
TS19: trunk mesenchyme	Strong	Regionally restricted	4E	Expression is in the mesenchyme underlying the hindgut.

Structure	Level	Pattern	Image	Note
TS22: axial skeleton tail region	Present	Regionally restricted	7A	Expression is in the intervertebral dics.

Structure	Level	Pattern	Image	Note
TS22: axial skeleton tail region	Present	Regionally restricted	7B	Expression is in the intervertebral dics.
TS22: tail paraxial mesenchyme	Present	Regionally restricted	7B	Expression is in the mesenchyme surrounding the muscle primordia.

Structure	Level	Pattern	Image	Note
TS20: hand	Present	Regionally restricted	8A	Expression is in the anterior proximal corner with weaker expression more posteriorly.

Structure	Level	Pattern	Image	Note
TS20: foot	Present	Regionally restricted	8B	Expression is in the anterior proximal corner with weaker expression more posteriorly.

Structure	Level	Pattern	Image	Note
TS22: hand	Strong	Regionally restricted	8C	Strong in the hand pads and weaker in mesenchyme between metacarpals and ventral to the primordia of the phalangeal bones.

Structure	Level	Pattern	Image	Note
TS22: foot	Strong	Regionally restricted	8D	Expression is anterior to the talus primordium, between the tarsals and metatarsals and ventral to the phalangeal primordia.

Structure	Level	Pattern	Image	Note
TS17: axial skeleton	Present	Regionally restricted	10E	Expression is detected up to the thoracic level but not in the lumbar, sacral, or caudal regions.
TS17: branchial pouch	Present		10E	Expression is the same as in wild-type.
TS17: nasal process	Present		10E	Expression is the same as in wild-type.