Probe:	pGli-3a
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS7: embryo	Absent

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Widespread	3A Gli3
TS11: embryo mesenchyme	Present	Widespread	3A Gli3
TS11: embryo endoderm	Absent		3A Gli3

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Widespread	3B Gli3
TS11: embryo mesenchyme	Present	Widespread	3B Gli3
TS11: extraembryonic mesenchyme	Present		3B Gli3
TS11: allantois	Strong		3B Gli3
TS11: amnion	Strong		3B Gli3
TS11: embryo endoderm	Absent		3B Gli3

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Widespread	3C Gli3
TS11: embryo mesenchyme	Present	Widespread	3C Gli3
TS11: allantois	Strong		3C Gli3
TS11: embryo endoderm	Absent		3C Gli3

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme derived from neural crest	Present		5I/J

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	5K/L	Expression was detected in the dorsal region of the developing neural tube.
TS13: somatopleure	Present		5K/L
TS13: somite	Present		5K/L

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present
TS13: trunk unsegmented mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS28: lung	Absent

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent

Structure	Level	Pattern	Image	Note
TS28: testis	Absent

Structure	Level	Pattern	Image	Note
TS19: metencephalon	Present	Regionally restricted	6A Gli3	Expression was detected throughout the roof region.
TS19: midbrain	Present	Regionally restricted	6A Gli3	Expression was detected throughout the roof region.
TS19: telencephalon	Present	Regionally restricted	6A Gli3	Expression was detected throughout the roof region.
TS19: gut mesenchyme	Present		6A Gli3
TS19: lung mesenchyme	Present		6A Gli3
TS19: myelencephalon ventricular layer	Present		6A Gli3
TS19: Rathke's pouch	Weak		6A Gli3
TS19: head	Present	Regionally restricted	6A Gli3	Expression was uniformly distributed in most craniofacial structures.
TS19: body wall	Present	Regionally restricted	6A Gli3	Expression was detected in the body wall of the thoracic and abdominal regions.
TS19: axial skeleton	Present		6A Gli3
TS19: limb	Present	Regionally restricted	6A Gli3	Expression was detected in the developing limb bones.

Structure	Level	Pattern	Image	Note
TS19: axial skeleton	Present		6B Gli3
TS19: limb mesenchyme	Present	Regionally restricted	6B Gli3	Expression was detected in the mesenchyme outside the blastema.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		7A Gli3
TS20: pons	Present		7A Gli3
TS20: telencephalon ventricular layer	Present		7A Gli3
TS20: midbrain	Present	Regionally restricted	7A Gli3	Expression was detected in the tectum.
TS20: hindbrain	Present	Regionally restricted	7A Gli3	Expression was detected in the pontine flexure.
TS20: head	Present	Regionally restricted	7A Gli3	Expression was uniformly distributed in most craniofacial structures.

Structure	Level	Pattern	Image	Note
TS22: forebrain ventricular layer	Present	Regionally restricted	7B Gli3	Expression was detected in the ventricular zone of both the anterior and posterior horn of the lateral ventricles.
TS22: hindbrain ventricular layer	Present	Regionally restricted	7B Gli3	Expression was detected in the ventricular zone of both the anterior and posterior horn of the lateral ventricles.

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	7C Gli3	Expression was diminished in the brain except in the cerebellum.
TS28: cerebellum	Present		7C Gli3

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted		Expression was detected in the dorsal ventricular zone.
TS15: branchial arch mesenchyme derived from neural crest	Present
TS15: optic vesicle	Present
TS15: head mesenchyme derived from neural crest	Present	Regionally restricted		Expression was detected in crest-derived facial mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: gut mesenchyme	Present		8A Gli3
TS22: lung mesenchyme	Present		8A Gli3
TS22: vertebral cartilage condensation	Present	Regionally restricted	8A Gli3	Expression was detected peripheral to the perichondrium.
TS22: heart	Absent		8A Gli3
TS22: liver	Absent		8A Gli3
TS22: perioptic mesenchyme	Present		8A Gli3	Expression was detected in the optic capsule.
TS22: nasal capsule	Present		8A Gli3
TS22: otic capsule	Present		8A Gli3
TS22: tooth	Present		8A Gli3
TS22: cranium	Present	Regionally restricted	8A Gli3	Expression was detected close to the base of the skull.
TS22: head mesenchyme	Present	Regionally restricted	8A Gli3	Expression was detected in the mesenchyme surrounding the whisker follicles.

Structure	Level	Pattern	Image	Note
TS22: genital tubercle	Present		8B Gli3
TS22: mesonephros	Present		8B Gli3
TS22: testis	Present		8B Gli3

Structure	Level	Pattern	Image	Note
TS22: otic capsule	Present		8C Gli3
TS22: tooth	Present		8C Gli3

Structure	Level	Pattern	Image	Note
TS22: lens	Present		8D Gli3
TS22: nasal capsule	Present		8D Gli3
TS22: optic stalk	Present		8D Gli3