Probe:	pGli-2
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS7: embryo	Absent

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Widespread	3A Gli2
TS11: embryo mesenchyme	Present	Widespread	3A Gli2
TS11: embryo endoderm	Absent		3A Gli2

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Widespread	3B Gli2
TS11: embryo mesenchyme	Present	Widespread	3B Gli2
TS11: extraembryonic mesenchyme	Present		3B Gli2
TS11: allantois	Strong		3B Gli2
TS11: amnion	Strong		3B Gli2
TS11: embryo endoderm	Absent		3B Gli2

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Widespread	3C Gli2
TS11: embryo mesenchyme	Present	Widespread	3C Gli2
TS11: allantois	Strong		3C Gli2
TS11: embryo endoderm	Absent		3C Gli2

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	5E/F	Expression was detected in the dorsal region of the developing neural tube.

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme derived from neural crest	Present		5G/H
TS13: somatopleure	Present		5G/H
TS13: somite	Present		5G/H

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present
TS13: trunk unsegmented mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS28: lung	Absent

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent

Structure	Level	Pattern	Image	Note
TS28: testis	Absent

Structure	Level	Pattern	Image	Note
TS19: metencephalon	Present	Regionally restricted	6A Gli2	Expression was detected throughout the roof region.
TS19: midbrain	Present	Regionally restricted	6A Gli2	Expression was detected throughout the roof region.
TS19: telencephalon	Present	Regionally restricted	6A Gli2	Expression was detected throughout the roof region.
TS19: gut mesenchyme	Present		6A Gli2
TS19: lung mesenchyme	Present		6A Gli2
TS19: myelencephalon ventricular layer	Present		6A Gli2
TS19: Rathke's pouch	Weak		6A Gli2
TS19: head	Present	Regionally restricted	6A Gli2	Expression was uniformly distributed in most craniofacial structures.
TS19: body wall	Present	Regionally restricted	6A Gli2	Expression was detected in the body wall of the thoracic and abdominal regions.
TS19: axial skeleton	Present		6A Gli2
TS19: limb	Present	Regionally restricted	6A Gli2	Expression was detected in the developing limb bones.

Structure	Level	Pattern	Image	Note
TS19: axial skeleton	Present		6B Gli2
TS19: limb mesenchyme	Present	Regionally restricted	6B Gli2	Expression was detected in the mesenchyme outside the blastema.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		7A Gli2
TS20: pons	Present		7A Gli2
TS20: telencephalon ventricular layer	Present		7A Gli2
TS20: hindbrain	Present	Regionally restricted	7A Gli2	Expression was detected in the pontine flexure.
TS20: midbrain	Present	Regionally restricted	7A Gli2	Expression was detected in the tectum.
TS20: head	Present	Regionally restricted	7A Gli2	Expression was uniformly distributed in most craniofacial structures.

Structure	Level	Pattern	Image	Note
TS22: brain	Weak		7B Gli2

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	7C Gli2	Expression was diminished in the brain except in the cerebellum.
TS28: cerebellum	Present		7C Gli2

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted		Expression was detected in the dorsal ventricular zone.
TS15: branchial arch mesenchyme derived from neural crest	Present
TS15: optic vesicle	Present
TS15: head mesenchyme derived from neural crest	Present	Regionally restricted		Expression was detected in crest-derived facial mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: gut mesenchyme	Present		8A Gli2
TS22: lung mesenchyme	Present		8A Gli2
TS22: vertebral cartilage condensation	Present	Regionally restricted	8A Gli2	Expression was found peripheral to the perichondrium.
TS22: heart	Absent		8A Gli2
TS22: liver	Absent		8A Gli2
TS22: perioptic mesenchyme	Present		8A Gli2	Expression was detected in the optic capsule.
TS22: nasal capsule	Present		8A Gli2
TS22: otic capsule	Present		8A Gli2
TS22: tooth	Present		8A Gli2
TS22: cranium	Present	Regionally restricted	8A Gli2	Expression was detected close to the base of the skull.
TS22: head mesenchyme	Present	Regionally restricted	8A Gli2	Expression was detected in the mesenchyme surrounding the whisker follicles.

Structure	Level	Pattern	Image	Note
TS22: genital tubercle	Present		8B Gli2
TS22: mesonephros	Present		8B Gli2
TS22: testis	Present		8B Gli2

Structure	Level	Pattern	Image	Note
TS22: otic capsule	Present		8C Gli2
TS22: tooth	Present		8C Gli2

Structure	Level	Pattern	Image	Note
TS22: lens	Present		8D Gli2
TS22: nasal capsule	Present		8D Gli2
TS22: optic stalk	Present		8D Gli2