Antibody:	anti- cyclin E (M-20) (sc-481)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		3A1 , 3A3
TS20: cortical preplate	Present		3A1 , 3A3

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		3E3 , 3E1
TS20: cortical preplate	Present		3E3 , 3E1

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		3F1 , 3F3
TS27: cerebellum external granule cell layer	Present		3F1 , 3F3

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		3J1 , 3J3
TS27: cerebellum external granule cell layer	Present		3J1 , 3J3

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Present		4A1 , 4A4	Expression was cytoplasmic in most postmitotic cortical neurons. There were some cells with intense nuclear staining.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		4B1 , 4B3	Staining in cortical neurons remained cytoplasmic. Nuclear staining remained weak.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		4C1 , 4C3	Intense nuclear staining appeared in some cortical neurons. Cytoplasmic staining remained intense in most neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		4D3 , 4D1	The number of cells with nuclear staining was increased. Cytoplasmic staining remained intense in most neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		4E1 , 4E3	Cytoplasmic staining remained intense in most neurons. Nuclear levels were markedly reduced in most neurons, although there were some neurons that retain intense nuclear staining.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		4F3 , 4F1	Intense staining was localized to the cytoplasm.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4G3 , 4G1	Intense nuclear staining appeared. Cytoplasmic staining remained intense in most neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4H1 , 4H3	Intense nuclear staining appeared. Cytoplasmic staining remained intense in most neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4I1 , 4I3	Cytoplasmic staining remained intense in most neurons. Nuclear levels were reduced in most neurons, although there were some neurons that retain intense nuclear staining.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		6A	The staining intensity in the nucleus was lower than that in the cytoplasm in most neurons, although there were some neurons that exhibited intense nuclear staining.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present		6B	The staining intensity in the nucleus was lower than that in the cytoplasm in most neurons, although there were some neurons that exhibited intense nuclear staining.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		6C	The staining intensity in the nucleus was lower than that in the cytoplasm in most neurons, although there were some neurons that exhibited intense nuclear staining.

Structure	Level	Pattern	Image	Note
TS28: thalamus	Present		6D	The staining intensity in the nucleus was lower than that in the cytoplasm in most neurons, although there were some neurons that exhibited intense nuclear staining.

Structure	Level	Pattern	Image	Note
TS28: pons	Present		6E	The staining intensity in the nucleus was lower than that in the cytoplasm in most neurons, although there were some neurons that exhibited intense nuclear staining.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present		6F	The staining intensity in the nucleus was lower than that in the cytoplasm in most neurons, although there were some neurons that exhibited intense nuclear staining.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		S1A1 , S1A3	Expression was not only in proliferating cells, but also in non-proliferating cells.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		S1E1 , S1E3	Expression was not only in proliferating cells, but also in non-proliferating cells.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		S2A3 , S2A1	Expression was not only in proliferating cells, but also in non-proliferating cells.
TS20: cortical preplate	Present		S2A3 , S2A1	Expression was not only in proliferating cells, but also in non-proliferating cells.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		S2B1 , S2B3	Expression was not only in proliferating cells, but also in non-proliferating cells.
TS27: cerebellum external granule cell layer	Present		S2B1 , S2B3	Expression was not only in proliferating cells, but also in non-proliferating cells.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		S4A	Intense staining was localized to the cytoplasm.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		S4C3 , S4D1 , S4C1 , S4D3	Intense staining was localized to the cytoplasm.