Probe:	Vasn cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		2A
TS13: neural tube	Present	Regionally restricted	2A	Expression is present at the midline.
TS13: lateral plate mesoderm	Present		2A	Expression is present in the lateral mesoderm and the caudal extremity.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	2B	Expression is present in the rhombic lip.
TS15: 1st branchial arch	Present		2B
TS15: forelimb bud	Present		2B
TS15: septum transversum	Present		2B
TS15: body cavity or lining	Present		2B	Expression is present in the lining of the coelomic cavity.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	2B'	Expression is present in the rhombic lip.

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		2B''
TS15: neural tube roof plate	Present		2B''

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Strong		2C
TS17: trunk mesenchyme	Strong		2C
TS17: 1st branchial arch	Strong		2C
TS17: 2nd branchial arch	Strong		2C
TS17: forelimb bud	Strong		2C
TS17: hindlimb bud	Strong		2C

Structure	Level	Pattern	Image	Note
TS19: axial skeleton	Present		2D	Expression is present in condensations along the forming vertebral axis.
TS19: branchial arch	Present		2D
TS19: embryo mesenchyme	Present		2D
TS19: forelimb bud	Present		2D
TS19: hindlimb bud	Present		2D