Probe:	Fzd7 cDNA4
Probe preparation:	Antisense RNA
Assay notes:	These data are obtained from optical projection tomography (OPT).

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present
TS19: 2nd branchial arch	Present
TS19: alimentary system	Present
TS19: diencephalon	Present
TS19: eye	Present
TS19: forelimb bud mesenchyme	Present
TS19: fronto-nasal process	Present
TS19: lung	Present
TS19: midbrain	Present
TS19: neural tube	Present
TS19: telencephalon	Present
TS19: inner ear	Present	Regionally restricted		Otic vesicle.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		5_Fzd7 first	Elevated expression in a ring in the proximal limb shaft
TS19: forelimb bud ectoderm	Present		5_Fzd7 first	Ventral ectoderm.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		5_Fzd7 second

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		5_Fzd7 third

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		5_Fzd7 fourth

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		5_Fzd7 fifth

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		5_Fzd7 sixth

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		5_Fzd7 seventh

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS19: midbrain ventricular layer	Present	Regionally restricted		In the ventral area, strongest at most dorsal extent of the domain.
TS19: telencephalon	Present	Regionally restricted		Dorso-lateral telencephalic vesicles.
TS19: diencephalon	Present	Regionally restricted		Two patches about mid way along diencephalon on d/v axis and toward but not at ventricular surface.
TS19: neural tube ventricular layer	Present	Regionally restricted		Ventral.
TS19: nasal process mesenchyme	Present	Regionally restricted		Most distal mesenchyme of frontonasal process- clear boundary especially around the nasal pits. Especially strong anterior medial mesenchyme around nasal pits.
TS19: 2nd branchial arch mesenchyme	Weak	Regionally restricted		Distal mesenchyme in 1st and 2nd branchial arch.
TS19: branchial arch mesenchyme	Weak	Regionally restricted		Distal mesenchyme in 1st and 2nd branchial arch.
TS19: forelimb bud mesenchyme	Present	Regionally restricted		Throughout mesenchyme but lowest at core proximal- elevated patches, strongest in proximal-ventral, also elevated central distal and proximal dorsal.
TS19: inner ear	Present	Regionally restricted		Otic vesicle, in posterior it is medial and about half way along AP extent is more lateral.
TS19: lens vesicle anterior epithelium	Present
TS19: lung epithelium	Present
TS19: pancreas primordium	Present
TS19: trunk	Present	Regionally restricted		Viscera, around branchial arch arteries (6th) and vitelline vein.
TS19: urogenital sinus	Present	Regionally restricted		Ventral point.
TS19: somite	Present	Regionally restricted		Expression is a extensive lateral stripes, strongest most dorsally. In cross section the domain is circular surrounding a core of non expression-lateral aspect of muscle mass.