Probe:	Nolz-1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Non-Uniform	1A	Areas of expression include strong expression at the forebrain-midbrain boundary.
TS13: future prosencephalon	Present	Non-Uniform	1A	Areas of expression include strong expression at the forebrain-midbrain boundary.
TS13: midbrain-hindbrain junction	Strong		1A
TS13: rhombomere 3	Strong		1A
TS13: rhombomere 5	Strong		1A
TS13: future hindbrain	Present		1A	Areas of expression include rhomobomeres 3 and 5, as well as more caudal expression.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Non-Uniform	1B	Areas of expression include strong expression at the forebrain-midbrain boundary.
TS15: future midbrain	Present	Non-Uniform	1B	Areas of expression include strong expression at the forebrain-midbrain boundary.
TS15: midbrain-hindbrain junction	Strong		1B
TS15: rhombomere 3	Strong		1B
TS15: rhombomere 5	Strong		1B
TS15: future hindbrain	Present		1B	Areas of expression include rhomobomeres 3 and 5, as well as more caudal expression.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Strong		1C
TS17: rhombomere 3	Strong		1C
TS17: rhombomere 5	Strong		1C
TS17: branchial arch	Present		1C
TS17: eye	Present		1C
TS17: forelimb bud	Present		1C

Structure	Level	Pattern	Image	Note
TS18: hindbrain	Present	Regionally restricted	2A , 2A inset	There is broad expression in the rhombomeres, except in the r1 region. Dinstinct clusters of expressing cells extend from r2-r5 and r6. There is expression in lateral areas as well.
TS18: rhombomere 1	Absent		2A
TS18: rhombomere 2	Present		2A
TS18: rhombomere 3	Present		2A
TS18: rhombomere 4	Present		2A
TS18: rhombomere 5	Present		2A
TS18: rhombomere 6	Present		2A
TS18: rhombomere 7	Present		2A
TS18: rhombomere 8	Present		2A
TS18: diencephalon	Present		2A
TS18: midbrain	Present		2A

Structure	Level	Pattern	Image	Note
TS18: hindbrain	Present	Regionally restricted	2B	There is broad expression in the rhombomeres, except in the r1 region. Expression is also present near the vestibulocochlear boundary cap. There is prominent expression in columns and clusters of cells elsewhere.
TS18: rhombomere 1	Absent		2B
TS18: rhombomere 2	Present		2B
TS18: rhombomere 3	Present		2B , 2B inset
TS18: rhombomere 4	Present		2B
TS18: rhombomere 5	Present		2B
TS18: rhombomere 6	Present		2B
TS18: rhombomere 7	Present		2B
TS18: rhombomere 8	Present		2B
TS18: branchial arch	Present		2B
TS18: midbrain	Present		2B

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	2C	There is prominent expression present in longitudinal columns.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	2D	Expression is present in the ventricular zone of rhombomeres 3 and the mantle zones of rhombomeres 3 and 5; it is not expressed in rhombomeres 1 and 4. There is a low level of expression in the ventricular zone of the rhombomere 2 region.

Structure	Level	Pattern	Image	Note
TS22: pons	Strong	Regionally restricted	2F	Expression is present in distinct domains, including the presumptive vestibular nucleus.
TS22: medulla oblongata	Present		2F

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	2G	Expression is present in longitudinal columns of reticular nuclei.
TS22: pons	Strong	Regionally restricted	2G	Expression is present in distinct domains, including the presumptive cochlear nucleus.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	2H	Expression is present in longitudinal columns of reticular nuclei.

Structure	Level	Pattern	Image	Note
TS19: hindbrain ventricular layer	Present		3A	Expression is present in the hindbrain alar plate.
TS19: hindbrain	Present	Regionally restricted	3A	Expression is present in the most lateral region of the pre-optic hindbrain, at a level anterior to and around the otic vesicle. It is not expressed in a domain in which the branchial motor neurons reside.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	3B	Expression is present in longitudinal columns.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	3C	Expression is present in longitudinal columns.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	3D	Expression is present in longitudinal columns.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		3D inset

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	3E	Expression is present in the most lateral region of the pre-optic hindbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	3F	Expression is present in longitudinal columns.

Structure	Level	Pattern	Image	Note
TS20: hindbrain ventricular layer	Present		3G
TS20: hindbrain	Present		3G	Areas of expression include the prospective pre-intermediate reticular zone.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present		3H	Areas of expression include the prepostitus hypoglossal nucleus area.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present		3H inset

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted		Expression is present in a number of nuclei, including: gigantocellular; inferior olive (principal and medial); lateral paragigantocellular nucleus; and hypoglossal. There is no expression in a number of other nuclei, detailed in the table.
TS26: pons	Present	Regionally restricted		There is strong expression in the dorsal cochlear nucleus and moderate expression in the ventral cochlear and prepositus hypoglossal nuclei.
TS26: brainstem	Present	Regionally restricted		There is moderate expression in the dorsal medullary reticular nucleus and weak in the caudal linear raphe, intermediate reticular zone, locus coerulus and spinal trigeminal nucleus. There is possible expression in the rostroventrolateral reticular nuc.

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present		4A
TS26: brainstem	Present		4A1 , 4A	Expression is present in the caudal linear nucleus raphe.
TS26: pons	Present		4A2 , 4A	No expression is seen in the trigeminal motor nucleus.
TS26: medulla oblongata	Strong		4A , 4A3 , 4A2	Expression is present in lateral paragigantocellular nucleus and the gigantocellular nucleus, as well as other cells.
TS26: brain	Present	Regionally restricted	4A	Expression is present in the neuroepthelium surrounding the dorsal part of aqueduct.

Structure	Level	Pattern	Image	Note
TS26: superior colliculus	Present		4B
TS26: medulla oblongata	Present		4B	Expression is present in the rostral medulla. It is not present in the nucleus of solitary tract.
TS26: midbrain	Weak		4B1 , 4B
TS26: pons	Present		4B3 , 4B	Expression is present in the laterodorsal and dorsal tegmental nuclei.
TS26: brainstem	Weak		4B3 , 4B	Expression is present in the locus coerulus.
TS26: brain	Present	Regionally restricted	4B	Expression is present in the neuroepthelium surrounding the dorsal part of aqueduct.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	4B4 , 4B	Expression is present in the prepositus hypoglossal nucleus.
TS26: cerebellum	Absent		4B , 4B4

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	4B , 4B6	Expression is present in both the dorsal and ventral cochlear nuclei.
TS26: medulla oblongata	Present	Regionally restricted	4B6 , 4B	Expression is present in the principal nucleus of inferior olive (pictured in the inset) and the ventral gigantoceulluar nucleus.
TS26: brainstem	Present	Scattered	4B , 4B6	Expression is present in the intermediate reticular zone. There is no expression in the parvocellular reticular nucleus.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	4C1	Expression is present in the vesticular nucleus.
TS26: medulla oblongata	Present	Regionally restricted	4C1 , 4C3	Expression is present in the medial nucleus of inferior olive and gigantocellular nucleus. There is no expression in the nucleus of solitary tract.
TS26: brainstem	Present	Scattered	4C3 , 4C1	Expression is present in the intermediate reticular zone. There is no expression in the dorsal paragigantocellular nucleus.

Structure	Level	Pattern	Image	Note
TS26: brainstem	Present	Regionally restricted	4C5 , 4C1	Expression is present in the spinal trigeminal nucleus.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted	4D2 , 4D1	Expression is present in somatic motor neurons, such as in the nucleus of the hypoglossal cranial nerve. Once such cell is pictured in the inset.
TS26: brainstem	Present	Regionally restricted	4D1	Experssion is present in the dorsal medullary reticular nucleus.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	5Bb2	Expression is present in the hindbrain, including in cells believed to be somatic motor neurons.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	5C	Expression is in areas where somatic motor neurons are present. It is not present in areas where branchial motor neurons are present.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	5E	Expression is not present in areas where branchial motor neurons are present. It is present elsewhere in the hindbrain.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	5G , 5H	Expression is present in somatic motor neurons. One of these expressing neurons from the medial part of the hindbrain at the level caudal to the optic vesicle is pictured in the inset.