Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75699
Albrecht AN, et al., The synpolydactyly homolog (spdh) mutation in the mouse -- a defect in patterning and growth of limb cartilage elements. Mech Dev. 2002 Mar;112(1-2):53-67
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5446465
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Gene symbol:
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Bmp2
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Gene name:
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bone morphogenetic protein 2
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Probe:
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BMP2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2 Bmp2 11.5 wt:
embryonic day 11.5
(more  )
Specimen
2 Bmp2 11.5 wt:
(close  )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: handplate mesenchyme |
Present |
Regionally restricted |
2_Bmp2 11.5 wt/wt
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Expression in area just below the apical ectodermal ridge (AER) and around the zone of polarizing activity (ZPA).
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Specimen
2 Bmp2 11.5 spdh/spdh:
(close )
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Genetic Background:
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B6C3Fe a/a-Hoxd13spdh/J
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Mutant Allele(s):
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Hoxd13spdh/Hoxd13spdh
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2 Bmp2 12.5 wt:
embryonic day 12.5
(more )
Specimen
2 Bmp2 12.5 wt:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2 Bmp2 12.5 spdh/spdh:
(close )
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Genetic Background:
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B6C3Fe a/a-Hoxd13spdh/J
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Mutant Allele(s):
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Hoxd13spdh/Hoxd13spdh
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hand mesenchyme |
Weak |
Regionally restricted |
2_Bmp2 12.5 spdh/spdh
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Expression is less intense, and expression corresponding to perichondrium of digital anlagen is diffuse, with irregular distance between individual digits.
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Specimen
2 Bmp2 13.5 wt:
embryonic day 13.5
(more )
Specimen
2 Bmp2 13.5 wt:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hand |
Present |
Regionally restricted |
2_Bmp2 13.5 wt/wt
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Expression between digital anlagen has completely separated.
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Specimen
2 Bmp2 13.5 spdh/spdh:
(close )
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Genetic Background:
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B6C3Fe a/a-Hoxd13spdh/J
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Mutant Allele(s):
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Hoxd13spdh/Hoxd13spdh
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hand mesenchyme |
Present |
Regionally restricted |
2_Bmp2 13.5 spdh/spdh
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Almost complete lack of separation between digital anlagen. Irregular width between stripes of expression.
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Analysis Tools