Probe:	Tbx15 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The authors reported that they used riboprobes derived from either the 5' end or the 3' end and obtained identical hybridization patterns with each; they did not specify which probe was used for the pictured results.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		5A
TS15: visceral organ system	Absent		5A	No expression was seen in the internal organs.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	5B	Expression is limited to the medial portion of the limb; it is excluded from the anterior and posterior margins.
TS17: hindlimb bud	Present	Regionally restricted	5B	Expression is limited to the medial portion of the limb; it is excluded from the anterior and posterior margins.
TS17: forelimb bud apical ectodermal ridge	Absent		5B
TS17: hindlimb bud apical ectodermal ridge	Absent		5B
TS17: 1st branchial arch	Present	Regionally restricted	5B	Expression is present on the dorsal, caudal edge.
TS17: 2nd branchial arch	Present	Regionally restricted	5B	Expression is present on the dorsal, caudal edge.
TS17: head	Strong	Regionally restricted	5B	There is a prominent line of expression dorsal to the developing eye (underlying the telencephalic bulges).
TS17: visceral organ system	Absent		5B	No expression was seen in the internal organs.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	5C	Expression is limited to the medial portion of the limb; it is excluded from the anterior and posterior margins.
TS19: hindlimb bud	Present	Regionally restricted	5C	Expression is limited to the medial portion of the limb; it is excluded from the anterior and posterior margins.
TS19: footplate apical ectodermal ridge	Absent		5C
TS19: handplate apical ectodermal ridge	Absent		5C
TS19: maxillary process	Present	Regionally restricted	5C	Expression is present on the lateral, caudal surface.
TS19: 1st branchial arch	Present	Regionally restricted	5C	Expression is present in the core of the mandibular process of the first arch.
TS19: outer ear	Present	Regionally restricted	5C	Expression outlines the rostral, dorsal edge of the hyomandibular cleft, which will become the external auditory meatus.
TS19: head	Strong	Regionally restricted	5C	There is a prominent expression surrounding most the eye; it is strongest at its dorsal edge.
TS19: visceral organ system	Absent		5C	No expression was seen in the internal organs.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	5D	Expression extends all down the flank. It is lost from the distal limb and becomes more medially restricted.
TS20: hindlimb	Present	Regionally restricted	5D	Expression extends all down the flank. It is lost from the distal limb and becomes more medially restricted.
TS20: mandibular process	Present	Regionally restricted	5D	Expression is present in portions surrounding the developing mouth.
TS20: maxillary process	Present	Regionally restricted	5D	Expression is present in portions surrounding the developing mouth.
TS20: head	Strong	Regionally restricted	5D	There is a prominent expression surrounding most the eye; it is strongest at its dorsal edge. There is a conspicuous absence of expression over the surface of the second branchial arch.
TS20: visceral organ system	Absent		5D	No expression was seen in the internal organs.

Structure	Level	Pattern	Image	Note
TS13: visceral organ system	Absent			No expression was seen in the internal organs.