Probe:	Raldh2 cDNA3
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Absent		2A	Expression was excluded from the dorsalmost otocyst epithelium.
TS17: endolymphatic appendage	Absent		2A

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2B	Expression was detected in the medial portion of the outer otocyst epithelium.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2C	Expression was detected in the medial portion of the outer otocyst epithelium.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2D	Expression became highly restricted in ventral sections of the otocyst.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Absent		2E	Expression was excluded from the anterior and ventralmost portion of the otocyst.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Absent		2F	Expression was excluded from the anterior and ventralmost portion of the otocyst.

Structure	Level	Pattern	Image	Note
TS19: saccule	Present	Regionally restricted	3A	Expression was detected in the cochlear duct.
TS19: semicircular canal	Present	Regionally restricted	3A	Expression was detected at the base of an outgrowing semicircular canal, opposite to the endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS19: cranial ganglion	Absent			None of the cranial nerve ganglia surrounding the otocyst displayed expression.

Structure	Level	Pattern	Image	Note
TS22: utricle epithelium	Present		3B
TS22: saccule mesenchyme	Present	Regionally restricted	3B	Expression was detected along the lateral edge of the saccule mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: cochlear ganglion	Absent		3C

Structure	Level	Pattern	Image	Note
TS22: cochlear duct mesenchyme	Present	Regionally restricted	3D	Expression was detected at the level of the basal turn of the cochlear mesenchyme, just above the edge of the cochlear canal towards the modiolus.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Present	Regionally restricted	3E	Expression was detected in the presumptive stria vascularis and the Reissner membrane.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Present	Regionally restricted	3F	Expression was detected in the presumptive stria vascularis and the Reissner membrane.

Structure	Level	Pattern	Image	Note
TS26: cochlea	Present		3G
TS26: inner ear vestibular component	Present		3G

Structure	Level	Pattern	Image	Note
TS26: cochlear duct	Present	Regionally restricted	3H	Expression was detected in the stria vascularis and the Reissner membrane.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct	Present	Regionally restricted	3I	Expression was detected in the stria vascularis and the Reissner membrane.

Structure	Level	Pattern	Image	Note
TS20: cochlear duct	Absent		4A	No expression was seen in this section plane of the presumptive cochlear canal.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct mesenchyme	Present	Regionally restricted	4C	Expression was restricted to the medial cochlear mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Present	Regionally restricted		Expression was detected in the stria vascularis and the Reissner membrane.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct	Present	Regionally restricted	4E	Expression was detected in the stria vascularis and the Reissner membrane.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct	Present	Regionally restricted	4G	Expression was detected in the stria vascularis and the Reissner membrane.