Probe:	Fgf17 cDNA4
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Fresh frozen sections were briefly fixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS13: midbrain-hindbrain junction	Present		4C	Expression was not as strong or broad as Fgf8.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	4F	Expression was detected in the anterior midline.
TS15: nose	Absent		4F	Expression was not detected in nasal epithelial primordium.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4I	Expression was detected in the septal neural epithelium and at the telencephalon-diencephalon junction.
TS19: diencephalon	Present	Regionally restricted	4I	Expression was restricted to the telencephalon-diencephalon junction. Expression was not seen in the ventral hypothalamus.
TS19: infundibular recess of 3rd ventricle	Absent		4I

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted		Expression was detected in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted		Expression was detected in the midline structures of the telencephalon.
TS20: diencephalon	Present	Regionally restricted		Expression was detected in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Present		5C	Expression was stronger and broader than Fgf8.

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Strong		5F	Expression was stronger and broader than Fgf8.

Structure	Level	Pattern	Image	Note
TS22: midbrain-hindbrain junction	Weak	Regionally restricted	5I	The expression domain narrowed and the signal was weaker compared to E12.5.

Structure	Level	Pattern	Image	Note
TS21: midbrain-hindbrain junction	Weak	Regionally restricted		The expression domain narrowed and the signal was weaker compared to E12.5.

Structure	Level	Pattern	Image	Note
TS23: midbrain-hindbrain junction	Absent

Structure	Level	Pattern	Image	Note
TS20: arch of aorta	Present		6D , 6B
TS20: innominate artery	Present		6D , 6B
TS20: dorsal aorta	Present		6F , 6B

Structure	Level	Pattern	Image	Note
TS23: arterial system	Present	Regionally restricted	6I	Expression was detected in the great arteries.
TS23: pericardium	Present		6I

Structure	Level	Pattern	Image	Note
TS24: descending thoracic aorta	Present		6L

Structure	Level	Pattern	Image	Note
TS24: pulmonary artery	Present		6O

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Present	Regionally restricted	7C	Expression was detected in the dorsal costal cartilage. Signal was strongest in the periosteum, but also detectable in costal chondrocytes.

Structure	Level	Pattern	Image	Note
TS23: rib	Present	Regionally restricted	7F	Expression was detected in the periosteum of the dorsal part of the rib.

Structure	Level	Pattern	Image	Note
TS23: rib	Absent		7H	Expression was absent from the ventral costal cartilage.

Structure	Level	Pattern	Image	Note
TS23: scapula	Present		7N

Structure	Level	Pattern	Image	Note
TS23: maxilla	Present
TS23: long bone	Absent

Structure	Level	Pattern	Image	Note
TS24: skeleton	Ambiguous			When ossification centers have been established, the signal became difficult to detect.