Probe:	Fgf8 probe49
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Fresh frozen sections were briefly fixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS13: midbrain-hindbrain junction	Present		4B	Expression was more broad and at a higher level than Fgf17.
TS13: future prosencephalon	Present	Regionally restricted	4B	Expression was detected in the anterior neural ridge.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	4E	Expression was detected in the anterior midline.
TS15: nose	Present	Regionally restricted	4E	Expression was detected in the nasal epithelial primordium.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4H	Expression was detected in the septal neural epithelium and at the telencephalon-diencephalon junction.
TS19: diencephalon	Present	Regionally restricted	4H	Expression was detected in the dorsal diencephalon midline, the ventral hypothalamus, and at the telencephalon-diencephalon junction.
TS19: infundibular recess of 3rd ventricle	Present		4H

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted		Expression was detected in the dorsal diencephalon midline and in the ventral hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted		Expression was detected in the midline structures of the telencephalon.
TS20: diencephalon	Present	Regionally restricted		Expression was detected in the dorsal diencephalon midline and in the ventral hypothalamus.

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Present		5B	Expression was not as strong or broad as Fgf17 at E11.5.

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Weak		5E	Expression was not as strong or broad as Fgf17.

Structure	Level	Pattern	Image	Note
TS22: midbrain-hindbrain junction	Absent		5H

Structure	Level	Pattern	Image	Note
TS21: midbrain-hindbrain junction	Absent

Structure	Level	Pattern	Image	Note
TS23: midbrain-hindbrain junction	Absent

Structure	Level	Pattern	Image	Note
TS20: arch of aorta	Weak		6C

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Weak		6E

Structure	Level	Pattern	Image	Note
TS23: arterial system	Present	Regionally restricted	6H	Expression was detected in the great arteries.
TS23: pericardium	Present		6H

Structure	Level	Pattern	Image	Note
TS24: descending thoracic aorta	Absent		6K

Structure	Level	Pattern	Image	Note
TS24: pulmonary artery	Weak		6N

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Present	Regionally restricted	7B	Expression was detected in the dorsal costal cartilage. Signal was strongest in the periosteum, but also detectable in costal chondrocytes.

Structure	Level	Pattern	Image	Note
TS23: rib	Present	Regionally restricted	7E	Expression was detected in the periosteum of the dorsal part of the rib.

Structure	Level	Pattern	Image	Note
TS23: rib	Absent		7G	Expression was absent from the ventral costal cartilage.

Structure	Level	Pattern	Image	Note
TS23: mandible	Present		7J

Structure	Level	Pattern	Image	Note
TS23: tibia	Present	Regionally restricted	7L	Expression was detected in the growth plate of developing long bones.

Structure	Level	Pattern	Image	Note
TS23: chondrocranium	Present	Regionally restricted		Expression was detected in the osteoblast compartment.

Structure	Level	Pattern	Image	Note
TS24: skeleton	Ambiguous			When ossification centers have been established, the signal became difficult to detect.