Probe:	Dlx-2 cDNA1
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Weak

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Present	Regionally restricted	1C/D	Expression extends from the apical ridge to the ventral surface of forelimb bud ectoderm.
TS15: forelimb bud presumptive apical ectodermal ridge	Present	Regionally restricted	1C/D	Expression extends from the apical ridge to the ventral surface of forelimb bud ectoderm.
TS15: forelimb bud mesenchyme	Absent		1C/D

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme	Present		1E/F
TS15: 2nd branchial arch mesenchyme	Present	Regionally restricted	1E/F	A core of mesenchymal cells do not show expression.
TS15: 3rd branchial arch mesenchyme	Present		1E/F
TS15: branchial arch mesenchyme	Present		1E/F	All four branchial arches show expression in their mesenchyme.
TS15: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	1E/F	Expression was detected in most of the mesenchymal cells in the lateral region of the first branchial arch mandibular component. A core of mesenchymal cells do not show expression.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch ectoderm	Present	Regionally restricted	1G/H	Expression was detected medially in the ectoderm of the first arch.
TS15: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	1G/H	In the mandibular component of the first arch, expression in the ectoderm is limited to a rostro-ventro-medial domain.
TS15: 1st branchial arch mandibular component mesenchyme	Present	Graded	1G/H	In the mandibular component of the first arch, expression in the mesenchyme follows a lateral-to-medial gradient.
TS15: embryo ectoderm	Present	Regionally restricted	1G/H	Expression was detected in a strip of ectoderm underlying the telencephalon. It extends rostrally from a region basal to the optic stalk and ends near the apex of the frontonasal prominence.
TS15: diencephalon	Present	Regionally restricted	1G/H	Expression was detected within the ventral thalamic domain.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mandibular component mesenchyme	Present		2A
TS14: 1st branchial arch maxillary component mesenchyme	Present		2A
TS14: 2nd branchial arch mesenchyme	Present		2A
TS14: embryo ectoderm	Present	Regionally restricted	2A	Expression was detected in a restricted domain of ectoderm along the frontonasal prominence.
TS14: head	Present	Regionally restricted	2A	Expression was noted in a patch of cells dorsal to the first branchial arch in the region of the neural tube. These cells are in the region of the primordia of the trigeminal ganglion and may be either placodal and/or neural crest derived cells.

Structure	Level	Pattern	Image	Note
TS14: otic pit	Present	Regionally restricted		Expression was detected in the ectoderm of the otic pit.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch mesenchyme	Present		2B
TS16: 2nd branchial arch mesenchyme	Present	Regionally restricted	2B	A core of mesenchymal cells do not show expression.
TS16: 3rd branchial arch mesenchyme	Present		2B
TS16: 4th branchial arch mesenchyme	Present		2B
TS16: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	2B	Expression was detected in most of the mesenchymal cells in the lateral region of the first branchial arch mandibular component. A core of mesenchymal cells do not show expression.
TS16: 1st branchial arch maxillary component ectoderm	Present	Regionally restricted	2B	Expression in the ectoderm is rostrally displaced compared to the expression in the underlying mesenchyme.

Structure	Level	Pattern	Image	Note
TS16: 2nd branchial arch mesenchyme	Present	Regionally restricted	2C	A core of mesenchymal cells do not show expression.
TS16: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	2C	Expression was detected in most of the mesenchymal cells in the lateral region of the first branchial arch mandibular component. A core of mesenchymal cells do not show expression.
TS16: 1st branchial arch maxillary component ectoderm	Present	Regionally restricted	2C	Expression in the ectoderm is rostrally displaced compared to the expression in the underlying mesenchyme.

Structure	Level	Pattern	Image	Note
TS16: 2nd branchial arch mesenchyme	Present	Regionally restricted	2D	A core of mesenchymal cells do not show expression.
TS16: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	2D	Expression was detected in most of the mesenchymal cells in the lateral region of the first branchial arch mandibular component. A core of mesenchymal cells do not show expression.
TS16: future forebrain	Present	Regionally restricted	2D	Expression was limited to lateral regions of the forebrain; medial regions showed no expression.
TS16: head mesenchyme derived from neural crest	Strong	Regionally restricted	2D	Expression was detected in a cluster of cells just rostral to the otic vesicle and dorsal to the second branchial arch.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	2E	In the mandibular component of the first arch, expression in the ectoderm is limited to a rostro-ventro-medial domain.
TS16: 1st branchial arch mandibular component mesenchyme	Present	Graded	2E	In the mandibular component of the first arch, expression in the mesenchyme follows a lateral-to-medial gradient.
TS16: arterial system	Present	Regionally restricted	2E	Expression was detected in the mesenchyme that lines the walls of the aortic arches.
TS16: embryo ectoderm	Present	Regionally restricted	2E	Expression was detected in a strip of ectoderm underlying the telencephalon. It extends rostrally from a region basal to the optic stalk and ends near the apex of the frontonasal prominence.
TS16: otocyst epithelium	Present	Regionally restricted	2E	Expression was detected in a dorsal portion of the otic vesicle epithelium.
TS16: future forebrain	Present	Regionally restricted	2E	Expression was limited to lateral regions of the forebrain; medial regions showed no expression.
TS16: head mesenchyme derived from neural crest	Present	Regionally restricted	2E	Expression in small clusters of cells, which may be neural crest, can be seen just rostral and caudal to the otic vesicle.
TS16: forelimb bud apical ectodermal ridge	Present	Regionally restricted	2E	Expression extends from the apical ridge to the ventral surface of forelimb bud ectoderm.
TS16: forelimb bud ectoderm	Present	Regionally restricted	2E	Expression extends from the apical ridge to the ventral surface of forelimb bud ectoderm.
TS16: forelimb bud mesenchyme	Absent		2E

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch ectoderm	Present	Regionally restricted	2F	Expression was detected medially in the ectoderm of the first arch.
TS16: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	2F	In the mandibular component of the first arch, expression in the ectoderm is limited to a rostro-ventro-medial domain.
TS16: 1st branchial arch mandibular component mesenchyme	Present	Graded	2F	In the mandibular component of the first arch, expression in the mesenchyme follows a lateral-to-medial gradient.
TS16: embryo ectoderm	Present	Regionally restricted	2F	Expression was detected in a strip of ectoderm underlying the telencephalon. It extends rostrally from a region basal to the optic stalk and ends near the apex of the frontonasal prominence.
TS16: otocyst epithelium	Present	Regionally restricted	2F	Expression was detected in a dorsal portion of the otic vesicle epithelium.
TS16: arterial system	Present	Regionally restricted	2F	Expression was detected in the mesenchyme that lines the walls of the aortic arches.
TS16: head mesenchyme derived from neural crest	Present	Regionally restricted	2F	Expression in small clusters of cells, which may be neural crest, can be seen just rostral and caudal to the otic vesicle.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	2G	In the mandibular component of the first arch, expression in the ectoderm is limited to a rostro-ventro-medial domain.
TS16: 1st branchial arch mandibular component mesenchyme	Present	Graded	2G	In the mandibular component of the first arch, expression in the mesenchyme follows a lateral-to-medial gradient.
TS16: embryo ectoderm	Present	Regionally restricted	2G	Expression was detected in a strip of ectoderm underlying the telencephalon. It extends rostrally from a region basal to the optic stalk and ends near the apex of the frontonasal prominence.
TS16: diencephalon	Present	Regionally restricted	2G	Expression extends from the region of the zona limitans intraparencephalica to the postoptic area.
TS16: telencephalon	Present	Regionally restricted	2G	Expression extends from the preoptic area along the floor of the cerebral vesicle.
TS16: forelimb bud apical ectodermal ridge	Present	Regionally restricted	2G	Expression extends from the apical ridge to the ventral surface of forelimb bud ectoderm.
TS16: forelimb bud ectoderm	Present	Regionally restricted	2G	Expression extends from the apical ridge to the ventral surface of forelimb bud ectoderm.
TS16: forelimb bud mesenchyme	Absent		2G
TS16: spinal ganglion	Weak		2G
TS16: genitourinary system	Weak	Regionally restricted	2G	Expression was detected in the pronephros and metanephros.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch ectoderm	Present	Regionally restricted	2H	Expression was detected medially in the ectoderm of the first arch.
TS16: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	2H	In the mandibular component of the first arch, expression in the ectoderm is limited to a rostro-ventro-medial domain.
TS16: 1st branchial arch mandibular component mesenchyme	Present	Graded	2H	In the mandibular component of the first arch, expression in the mesenchyme follows a lateral-to-medial gradient.
TS16: embryo ectoderm	Present	Regionally restricted	2H	Expression was detected in a strip of ectoderm underlying the telencephalon. It extends rostrally from a region basal to the optic stalk and ends near the apex of the frontonasal prominence.
TS16: diencephalon	Present	Regionally restricted	2H	Expression extends from the region of the zona limitans intraparencephalica to the postoptic area.
TS16: telencephalon	Present	Regionally restricted	2H	Expression extends from the preoptic area along the floor of the cerebral vesicle.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch maxillary component ectoderm	Present	Regionally restricted	3A	Expression in the ectoderm of the maxillary processes extends into the olfactory pit.
TS17: nasal pit	Present	Regionally restricted	3A	Expression in the ectoderm of the maxillary processes extends into the olfactory pit. Ectodermal expression is also seen in a sharply defined domain at the dorsal lip of the olfactory pit.
TS17: head mesenchyme derived from neural crest	Present	Regionally restricted	3A	A cluster of cells was detected just dorsal to the maxillary branch of the first branchial arch and just caudal to the eye. The dorsal-lateral surface of the head is lined with positive cells.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	3B , 3C	Expression was detected in the medial part of the mandibular branch of the first arch.
TS17: spinal ganglion	Present		3B
TS17: diencephalon	Present	Regionally restricted	3C , 3B	Expression was detected in the ventral thalamus, posterior entopeduncular area, hypothalamic cell cord, and the primodia of the suprachiasmatic area. The domain stops at the boundary with the postoptic area.
TS17: telencephalon	Present	Regionally restricted	3C , 3B	Expression extends from the preoptic area along the floor of the cerebral vesicle under the primordia of the medial and lateral ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	3D	Expression was detected in the medial part of the mandibular branch of the first arch.
TS17: spinal ganglion	Present		3D

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present	Regionally restricted	3E , 3F	Expression was limited to two discontinuous domains in the forebrain.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4A/B	Laterally, only the domain in the telencephalon is noted. Expression was in the lateral and medial ganglionic eminences (LGE,MGE). Caudal boundary between the MGE and caudal ganglionic eminence; rostral boundary between the LGE and cerebral cortex.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4C/D	Expression was in the septum, medial ganglionic eminence, anterior entopeduncular area and preoptic area.
TS19: diencephalon	Present	Regionally restricted	4C/D	Expression was in the suprachiasmatic area, hypothalamic cell cord, posterior entopeduncular area and ventral thalamus. Caudal boundary was at the zone limitans. The negative region consisted of the eminentia thalami and several hypothalamic primordia.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4E/F	Expression was in the septum, medial ganglionic eminence, anterior entopeduncular area and preoptic area.
TS19: diencephalon	Present	Regionally restricted	4E/F	Expression was in the suprachiasmatic area, hypothalamic cell cord, posterior entopeduncular area and ventral thalamus. Caudal boundary was at the zone limitans. The negative region consisted of the eminentia thalami and several hypothalamic primordia.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4G/H	In the most medial section, expression was only in the rostral domain.