Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Present	Widespread	2a	Expression was detected in both the future dorsal ectoderm and in the neuroepithelium.
TS12: embryo mesenchyme	Present	Widespread	2a	Expression was detected in most mesoderm.
TS12: extraembryonic mesenchyme	Present		2a

Structure	Level	Pattern	Image	Note
TS13: embryo	Strong	Regionally restricted	2b	Expression was strong in the posterior region of the embryo.
TS13: amnion	Strong		2b
TS13: neural ectoderm	Present	Regionally restricted	2b	Expression is present throughout the neural tube, from the prospective forebrain to its caudalmost part.
TS13: future prosencephalon	Present	Regionally restricted	2b	Expression was detected in the neural tube.
TS13: embryo mesenchyme	Present	Regionally restricted	2b	Expression was detected in the posterior mesoderm.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from somatopleure	Strong		2c
TS15: mesenchyme derived from splanchnopleure	Strong		2c
TS15: neural tube	Strong		2c	The entire open neuroepithelium expresses the reporter.
TS15: hindgut	Strong	Regionally restricted	2c	Expression was strong in the ventral part of the hindgut.
TS15: trunk paraxial mesenchyme	Present	Single cells	2c	Expression was detected in some cells in the paraxial presomitic mesoderm.
TS15: embryo ectoderm	Present		2c
TS15: notochord	Present		2c

Structure	Level	Pattern	Image	Note
TS15: gut	Weak		2d	In a more anterior domain compared to the section in Fig. 2C, expression in the splanchnopleure and gut was weakened.
TS15: mesenchyme derived from splanchnopleure	Weak		2d	In a more anterior domain compared to the section in Fig. 2C, expression in the splanchnopleure and gut was weakened.
TS15: mesenchyme derived from somatopleure	Present		2d
TS15: paraxial mesenchyme	Absent		2d
TS15: somite	Absent		2d
TS15: neural tube	Present	Regionally restricted	2d	Expression was detected in the dorsal-most portion of the more mature neural tube.
TS15: embryo mesenchyme	Present	Regionally restricted	2d	Expression was detected in the intermediate mesoderm.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	2e	Expression was detected in neural crest cells as they leave the dorsal neural tube.
TS15: forelimb bud	Present		2e	Expression was detected in the whole forelimb bud.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	2f	Expression was detected in migrating cephalic neural crest cells. Strong migration was noted from rhombomeres 2,4 and 6 with weaker and delayed migration from rhombomeres 3 and 5.
TS15: otocyst	Present		2f
TS15: rhombomere 2	Present		2f
TS15: rhombomere 3	Present		2f
TS15: rhombomere 4	Present		2f
TS15: rhombomere 5	Present		2f
TS15: rhombomere 6	Present		2f

Structure	Level	Pattern	Image	Note
TS15: rhombomere 2	Weak		2f inset
TS15: rhombomere 4	Weak		2f inset
TS15: rhombomere 6	Weak		2f inset
TS15: rhombomere 3	Strong		2f inset
TS15: rhombomere 5	Strong		2f inset

Structure	Level	Pattern	Image	Note
TS16: gut	Absent

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch maxillary component	Strong		3a H	No difference in expression pattern was noted between heterozygous and homozygous embryos.
TS16: forelimb bud	Strong		3a H	No difference in expression pattern was noted between heterozygous and homozygous embryos.
TS16: hindlimb bud	Strong		3a H	No difference in expression pattern was noted between heterozygous and homozygous embryos.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch maxillary component	Strong		3a h	No difference in expression pattern was noted between heterozygous and homozygous embryos.
TS16: forelimb bud	Strong		3a h	No difference in expression pattern was noted between heterozygous and homozygous embryos.
TS16: hindlimb bud	Strong		3a h	No difference in expression pattern was noted between heterozygous and homozygous embryos.

Structure	Level	Pattern	Image	Note
TS21: mammary gland primordium	Present		3d h	Expression was detected in the mammary gland in males and females.
TS21: body wall mesenchyme	Present	Regionally restricted	3d h	Expression in the lateral mesoderm is restricted to the region of the body wall where closure is still taking place.
TS21: embryo	Present		3d h	The expression tended to be stronger in homozygotes than in heterozygotes.
TS21: blood vessel	Present		3d h
TS21: outer ear	Present		3d h

Structure	Level	Pattern	Image	Note
TS21: mammary gland primordium	Present		3d H	Expression was detected in the mammary gland in males and females.
TS21: body wall mesenchyme	Present	Regionally restricted	3d H	Expression in the lateral mesoderm is restricted to the region of the body wall where closure is still taking place.
TS21: embryo	Present		3d H	The expression tended to be stronger in homozygotes than in heterozygotes.
TS21: blood vessel	Present		3d H
TS21: outer ear	Present		3d H

Structure	Level	Pattern	Image	Note
TS21: outer ear	Present		3e h

Structure	Level	Pattern	Image	Note
TS21: outer ear	Present		3e H

Structure	Level	Pattern	Image	Note
TS17: limb	Strong		4a	Expression was detected widely in the progress zone.
TS17: embryo	Present	Regionally restricted	4a	Expression was detected in the region neighboring the neural tube.
TS17: 1st branchial arch	Present		4a