Probe:	Evi1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: head	Strong	Regionally restricted	8A	High levels of expression limited to anterior of primary headfolds.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		8B
TS15: olfactory placode	Present	Regionally restricted	8B	Authors report expression in the nasal placode, and tissues of buccal cavity, particularly in nasal pits.
TS15: forelimb bud	Strong		8B
TS15: mesonephros	Strong		8B
TS15: 1st branchial arch mandibular component mesenchyme	Strong	Regionally restricted	8B	Highest branchial arch expression in posterior side of maxillary arch and anterior side of mandibular arch.
TS15: 1st branchial arch maxillary component mesenchyme	Strong	Regionally restricted	8B	Highest branchial arch expression in posterior side of maxillary arch and anterior side of mandibular arch.
TS15: 2nd branchial arch	Present	Uniform	8B

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		8C
TS17: 2nd branchial arch	Present		8C
TS17: forebrain	Present		8C
TS17: forelimb bud	Present		8C
TS17: hindlimb bud	Present		8C
TS17: nasal pit	Present		8C
TS17: mesonephros	Weak		8C
TS17: dorsal root ganglion	Present		8C	Expression in the trunk region lateral to the neural tube at the dorsal extreme of and between all somites. Pattern of expression corresponds with dorsal root gangla.
TS17: hindbrain	Present	Regionally restricted	8C	Two regions lateral to the 2nd branchial arch, near the otic vesicle. Medial region may be an ectodermal (epibranchial) placode contributing to placodal neurons of cranial ganglia.
TS17: rhombomere 4	Present		8C

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		8D
TS17: forelimb bud	Present		8D
TS17: rhombomere 4	Present		8D

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		8E	Expression of an alternatively spliced form limited to the limb bud.