Probe:	Hox3.6 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	6B	Somewhat diffuse anterior expression boundary at the level of the 13th thoracic vertebra, C13

Structure	Level	Pattern	Image	Note
TS17: trunk	Present	Regionally restricted	7A/A'	Expression restricted to body region posterior to the umbilical cord.
TS17: neural tube	Present	Regionally restricted	7A/A'	Expression in the posterior neural tube
TS17: hindlimb bud	Present	Regionally restricted	7A/A'	Expression in a cluster of cells in the proximal hindlimb bud.
TS17: extraembryonic component	Absent		7A/A'	No expression in the umbilical cord.

Structure	Level	Pattern	Image	Note
TS15: embryo	Strong	Regionally restricted	7B/B'	Strong, relatively uniform expression over the entire posterior region. Distinct anterior boundary of expression at the level of the umbilical cord.
TS15: tail	Strong		7B/B'

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	8B/B'	Expression restricted to posterior regions including posterior neural tube and lateral mesodermal tissue. No expression in anterior regions, including the anterior neural tube. No expression in a region ventral to the dorsal aorta.
TS15: intraembryonic coelom	Absent		8B/B'

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	8D/D'	Expression restricted to posterior regions including posterior neural tube and posterior mesodermal tissue.
TS17: trunk mesenchyme	Present	Regionally restricted	8D/D'	Expression restricted to posterior regions including posterior neural tube and posterior mesodermal tissue.
TS17: hindlimb bud	Present		8D/D'
TS17: dorsal aorta	Absent		8D/D'	No expression over the dorsal aorta or the undefined subregion located next to it in the ventro-lateral direction

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	9A/A'	Strongest expression in antero-proximal region of hindlimb bud, decreasing distally. No expression over distal half of limb bud.
TS20: body wall	Present	Regionally restricted	9A/A'	Expression in the ventro-lateral body wall.
TS20: tail	Present	Widespread	9A/A'	Authors report expression throughout the tail region

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	9B/B'	Weaker expression over the postero-proximal hindlimb bud in comparison with the antero-proximal region. No expression over the posterior mid-region of the limb bud.
TS20: trunk mesenchyme	Present	Regionally restricted	9B/B'	Expression in the lateral mesoderm extends dorsally from the limb bud. Uniform expression in the surrounding mesodermal tissue.
TS20: metanephros	Present		9B/B'
TS20: hindgut	Absent		9B/B'
TS20: pancreas	Absent		9B/B'

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	9C/C'	Expression starts to appear over the spinal cord. Domain of maximum expression in the posterior neural tube in a subregion near the hindlimb bud, gradually decreasing toward the tail.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present	Regionally restricted	9D/D'	Expression in the posterior spinal cord restricted to a ventral subregion of the mantle layer corresponding to the ventral horns.
TS20: spinal cord ventricular layer	Absent		9D/D'
TS20: spinal ganglion	Absent		9D/D'