Probe:	Zfp462 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	4A	Expression in the dentate gyrus and ventricular zone of the hippocampus.
TS12: extraembryonic component	Absent		4A

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	4F	Expression in the anterior ridge of neurectoderm.
TS12: extraembryonic component	Absent		4F

Structure	Level	Pattern	Image	Note
TS12: head	Present	Regionally restricted	4B	Authors report expression in head folds and neural groove.
TS12: neural ectoderm	Present	Regionally restricted	4B	Authors report expression in head folds and neural groove.

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Present		4G
TS13: future prosencephalon	Present		4G

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		4C

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		4H
TS15: future midbrain	Present		4H
TS15: future spinal cord	Present		4H
TS15: otocyst	Present		4H

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Present		4K
TS16: 2nd branchial arch	Present		4K
TS16: otocyst	Present		4K

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		4D
TS17: telencephalon	Present		4D

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		4I
TS17: future spinal cord	Present		4I
TS17: hindbrain	Present		4I
TS17: otocyst	Present		4I

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		4L
TS17: 2nd branchial arch	Present		4L
TS17: forelimb bud	Present		4L
TS17: telencephalon	Present		4L

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		4E	Authors state that expression is in the same structures as that seen at E10.5.
TS19: hindlimb bud	Present		4E	Authors state that expression is in the same structures as that seen at E10.5.
TS19: midbrain-hindbrain junction	Present		4E	Authors state that expression is in the same structures as that seen at E10.5.
TS19: telencephalon	Present		4E	Authors state that expression is in the same structures as that seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		4J	Authors state that expression is in the same structures as that seen at E10.5.
TS19: future spinal cord	Present		4J	Authors state that expression is in the same structures as that seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: dermomyotome	Present		4M
TS19: forelimb bud	Present		4M
TS19: hindlimb bud	Present		4M

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	5A	Expression in the anterior neurectoderm.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		5B
TS17: somite	Present		5B
TS17: future spinal cord	Present	Regionally restricted	5B	Expression in the dorsal spinal cord.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5C	Expression in muscle precursor cells.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	5D	Expression in the preplate.
TS19: telencephalon ventricular layer	Present		5D

Structure	Level	Pattern	Image	Note
TS19: inner ear	Present	Regionally restricted	5E	Authors state that expression is in the same structures as that seen at E10.5.
TS19: facial ganglion	Present		5E
TS19: telencephalon	Present		5E

Structure	Level	Pattern	Image	Note
TS19: glossopharyngeal ganglion	Present		5F
TS19: vagus ganglion	Present		5F
TS19: hindbrain	Present	Regionally restricted	5F	Expression in the caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	5G	Expression in muscle progenitors in the forelimb.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		6A

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted	6D
TS22: telencephalon septum	Present	Regionally restricted	6D

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Graded	6B

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted	6E	Strongest expression in caudal and medial cortical structures.

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Graded	6C

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Present	Regionally restricted	6F	Strongest expression in caudal and medial cortical structures.
TS26: telencephalon	Present	Regionally restricted	6F	Strongest expression in caudal and medial cortical structures.
TS26: main olfactory bulb	Present		6F

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Graded	7A	Caudal (high) to rostral (low) gradient of expression in all layers of cerebral cortex.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Present		7C
TS23: cerebral cortex subventricular zone	Present		7C
TS23: cortical plate	Present		7C
TS23: cerebral cortex ventricular layer	Weak		7C	Expression downregulated in ventricular zone.
TS23: hippocampus	Present	Regionally restricted	7C	Expression in the subiculum and CA1 field.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Present		8_E14.5 far left
TS22: cerebral cortex ventricular layer	Present		8_E14.5 far left
TS22: telencephalon septum	Present	Regionally restricted	8_E14.5 far left	Expression in ventricular zone of septal area.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		8_E14.5 left
TS22: hippocampus	Present		8_E14.5 left
TS22: telencephalon	Present	Regionally restricted	8_E14.5 left	Authors report expression in the preoptic region.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		8_E14.5 right
TS22: hippocampus	Present		8_E14.5 right
TS22: thalamus	Present	Regionally restricted	8_E14.5 right	Expression in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Present		8_E14.5 far right
TS22: cerebral cortex ventricular layer	Present		8_E14.5 far right
TS22: cortical plate	Present		8_E14.5 far right
TS22: thalamus	Present	Regionally restricted	8_E14.5 far right	Expression in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Present		8_E16.5 far left
TS24: basal ganglia	Present	Regionally restricted	8_E16.5 far left	Expression in the basal ganglia (striatal subventricular zone).

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Strong	Graded	8_E16.5 left
TS24: cerebral cortex subventricular zone	Present		8_E16.5 left
TS24: corpus striatum	Present		8_E16.5 left
TS24: cortical plate	Present		8_E16.5 left
TS24: basal ganglia	Present	Regionally restricted	8_E16.5 left	Expression in basal ganglia (pallidum).

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present	Graded	8_E16.5 right	Slight gradient of expression with higher levels in caudal and medial cortical structures.
TS24: cerebral cortex marginal layer	Present		8_E16.5 right
TS24: cortical plate	Present		8_E16.5 right
TS24: hippocampus	Present	Regionally restricted	8_E16.5 right	Expression in subiculum and CA1 field.
TS24: thalamus	Present	Regionally restricted	8_E16.5 right	Expression in the dorsal thalamus

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Present	Graded	8_E16.5 far right	High expression in marginal zone; graded expression with higher levels in caudal and medial cortical structures.
TS24: hippocampus	Present	Regionally restricted	8_E16.5 far right	Expression in subiculum and CA1 field.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		8_E18.5 far left	Authors state that expression in cerebral cortex subventricular zone is not clearly graded.
TS26: basal ganglia	Present	Regionally restricted	8_E18.5 far left	Expression in striatal ventricular zone and basal ganglia (striatum and pallidum).
TS26: corpus striatum	Present	Regionally restricted	8_E18.5 far left	Expression in striatal ventricular zone and basal ganglia (striatum and pallidum).

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present		8_E18.5 left
TS26: hippocampus CA1	Present		8_E18.5 left
TS26: hippocampus	Present	Regionally restricted	8_E18.5 left	Expression in the subiculum
TS26: thalamus	Present	Regionally restricted	8_E18.5 left	Expression ventrolateral nucleus.
TS26: zona incerta	Present		8_E18.5 left
TS26: prethalamus	Present		8_E18.5 left	Expression in nucleus reuiens.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Strong	Graded	8_E18.5 right	Strong expression in marginal zone; highly graded expression with maximal levels in caudal and medial cortical structures.
TS26: cortical plate	Present		8_E18.5 right
TS26: hippocampus	Present	Regionally restricted	8_E18.5 right	Expression in the subiculum.

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	8_E18.5 far right	Strong expression in subiculum.
TS26: cortical plate	Strong		8_E18.5 far right