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Gene Expression Data
Assay Details
Assay
Reference: J:42028 Seitanidou T, et al., Krox-20 is a key regulator of rhombomere-specific gene expression in the developing hindbrain. Mech Dev. 1997 Jul;65(1-2):31-42
Assay type: RNA in situ
MGI Accession ID: MGI:5436405
Gene symbol: Hoxb3
Gene name: homeobox B3
Probe: Hox-2.7 probe2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3A: embryonic day 8.5 (more )
Note: 8 somites.
Structure Level Pattern Image Note
TS13: rhombomere 4 Absent 3A
TS13: rhombomere 5 Strong 3A
TS13: future spinal cord Present Non-Uniform 3A Expression was not uniform along the rostrocaudal axis. Expression was higher in the prospective posterior spinal cord, where the tube was not yet closed.
Specimen 3B: embryonic day 8.5; Egr2tm1Pch/Egr2tm1Pch (more )
Note: 8 somites.
Structure Level Pattern Image Note
TS13: future spinal cord Present Regionally restricted 3B High relative level expression in the anterior domain was not observed.
TS13: rhombomere 5 Weak 3B
TS13: future hindbrain Weak Regionally restricted 3B Low level expression persisted in the posterior hindbrain.
Specimen 3C: embryonic day 8.5; Egr2tm1Pch/Egr2tm1Pch (more )
Note: 8 somites. Also stained for Hoxb1 (assay MGI:5436406).
Structure Level Pattern Image Note
TS13: rhombomere 5 Weak 3C A low level of expression was maintained in prospective rhombomere 5.
Specimen 3D: embryonic day 8.5 (more )
Note: 10-12 somites.
Structure Level Pattern Image Note
TS13: rhombomere 4 Absent 3D
TS13: rhombomere 5 Strong 3D
TS13: future spinal cord Present Non-Uniform 3D Expression was not uniform along the rostrocaudal axis. Expression was higher in the prospective posterior spinal cord, where the tube was not yet closed.
Specimen 3E: embryonic day 8.5; Egr2tm1Pch/Egr2tm1Pch (more )
Note: 10-12 somites.
Structure Level Pattern Image Note
TS13: future spinal cord Present Regionally restricted 3E High relative level expression in the anterior domain was not observed.
TS13: rhombomere 5 Weak 3E
TS13: future hindbrain Weak Regionally restricted 3E Lower level expression persisted in the posterior hindbrain.
Specimen 3F: embryonic day 8.5; Egr2tm1Pch/Egr2tm1Pch (more )
Note: 10-12 somites. Also stained for Hoxb1 (assay MGI:5436406).
Structure Level Pattern Image Note
TS13: rhombomere 5 Weak 3F A low level of expression was maintained in prospective rhombomere 5.
Specimen not shown: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: rhombomere 4 Absent
TS15: rhombomere 5 Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory