Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:96463
Moraes F, et al., Tbx1 is required for proper neural crest migration and to stabilize spatial patterns during middle and inner ear development. Mech Dev. 2005 Feb;122(2):199-212
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5435575
|
|
Gene symbol:
|
Pax2
|
|
Gene name:
|
paired box 2
|
|
Probe:
|
Pax2 probe16
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Assay notes:
|
After whole-mount staining, embryos were post-fixed in 4% paraformaldehyde/0.2% glutaraldehyde before sectioning.
|
|
|
Results
|
Specimen
4E:
embryonic day 9.5
(more  )
Specimen
4E:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst |
Present |
Regionally restricted |
4E
|
Expression was detected in the ventro-medial wall of the otocyst.
|
Specimen
4F:
embryonic day 9.5
(more )
Specimen
4F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Gelatin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst |
Present |
Regionally restricted |
4F
|
Expression was detected in the ventro-medial wall of the otocyst.
|
Specimen
4G:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Tbx1tm1Pa/Tbx1tm1Pa
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst |
Present |
Regionally restricted |
4G
|
Expression was detected in the ventro-medial wall of the otocyst as in wild type. Expression appeared weaker in caudal areas of the mutant vesicle.
|
Specimen
4H:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Tbx1tm1Pa/Tbx1tm1Pa
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Gelatin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst |
Present |
Regionally restricted |
4H
|
Expression was detected in the ventro-medial wall of the otocyst as in wild type.
|
Specimen
5A:
embryonic day 10.5
(more )
Specimen
5A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5A
|
Expression was detected in ventro-medial and caudal areas of the otic vesicle.
|
Specimen
5B:
embryonic day 10.5
(more )
Specimen
5B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Gelatin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5B
|
Expression was detected in ventro-medial and caudal areas of the otic vesicle.
|
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Tbx1tm1Pa/Tbx1tm1Pa
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Widespread |
5C
|
In the mutant, expression was expanded to a wider area of the otic vesicle, including the lateral and rostral walls.
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Tbx1tm1Pa/Tbx1tm1Pa
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Gelatin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Widespread |
5D
|
In the mutant, expression was expanded to a wider area of the otic vesicle, including the lateral and rostral walls.
|
|
Analysis Tools