Probe:	Nedd9 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted		Expression was high in dorsal and ventral domains in the trunk neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	1C	Expression was strong in the ventricular Nestin+ progenitor domain and excluded from the Tubb3+ cells at the emerging marginal zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	1F	Expression was confined to the ventricular zone (Nestin+/Tubb3-) and excluded from the post-mitotic neurons in the marginal zone and neuronal progenitor cells in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	1I , 1L	Expression was detected in radial glia progenitors of the neuroepithelium (Nestin+/Sox2+). It was mutually exclusive with with Tubb3 (Tuj1) expression.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2A	Expression was detected in the neuroepithelium of the ventral regions largely overlapping with the Neurog2 expression domain in the dorsoventral axis. Expression extends ventrally up to the floor plate and was not detected in the emerging marginal zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2D	Expression was detected in 2 discrete domains. Expression was detected in the ventricular zone. No expression in committed progenitors of the intermediate zone.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	2F	Expression was uniformly distributed in the dorso-ventral axis in the ventricular zone. Expression was detected close to the central canal.
TS20: spinal cord ventricular layer	Present		2F

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	3D	Expression was detected in the apical ventral midbrain neuroepithelium.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	3E	Expression was detected in the apical ventral midbrain neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	3H	Expression was detected in the ventricular zone of the ventral midbrain.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Strong	Regionally restricted	4D	Expression was detected in the ventricular zone of the cerebellum.

Structure	Level	Pattern	Image	Note
TS16: primitive ventricle cardiac muscle	Present	Regionally restricted	5A	Expression was detected in cardiomyocyte progenitors of the heart ventricle.

Structure	Level	Pattern	Image	Note
TS16: primitive ventricle cardiac muscle	Present	Regionally restricted	5B	Expression was detected in cardiomyocyte progenitors of the heart ventricle.

Structure	Level	Pattern	Image	Note
TS17: optic cup inner layer	Present	Regionally restricted	5D	Expression was detected in the prospective retina in the area corresponding to the ventricular zone of the neuroepithelium.
TS17: nasal pit epithelium	Present		5D	Expression was detected in olfactory pit epithelium.

Structure	Level	Pattern	Image	Note
TS17: nasal pit epithelium	Present		5E	Expression was detected in olfactory pit epithelium.

Structure	Level	Pattern	Image	Note
TS20: gonad germinal epithelium	Present		6A	Expression was detected in the gonad somatic epithelium.
TS20: mesonephros	Present		6A

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Absent		6E
TS20: cartilage	Present	Regionally restricted	6E	Expression was detected in progenitor cells of the prospective cartilage at trunk level.

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Absent		6G
TS20: cartilage	Present	Regionally restricted	6G	Expression was detected in progenitor cells of the prospective cartilage at cranial level.
TS20: blood vessel	Present	Regionally restricted	6G	Expression was detected in endothelial cells of vessels in the trigeminal ganglia.

Structure	Level	Pattern	Image	Note
TS17: gut epithelium	Present		7A

Structure	Level	Pattern	Image	Note
TS20: gut epithelium	Present		7B
TS20: respiratory system epithelium	Present		7B

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	7G	Expression was detected in meninges of posterior encephalic regions.

Structure	Level	Pattern	Image	Note
TS20: urinary system	Present	Regionally restricted	7H	Expression was detected in the urinary tract.

Structure	Level	Pattern	Image	Note
TS28: male accessory gland	Present		S1A

Structure	Level	Pattern	Image	Note
TS28: endometrium	Present	Regionally restricted	S1B	Expression was detected in glands of the endometrium.

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Absent		S1C	No signal was observed in cardiomyocytes.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Absent		S1D

Structure	Level	Pattern	Image	Note
TS28: colon	Absent		S1E

Structure	Level	Pattern	Image	Note
TS20: liver	Absent		S1F

Structure	Level	Pattern	Image	Note
TS28: adipose tissue	Absent
TS28: cerebellum	Absent
TS28: heart	Absent
TS28: hippocampus	Absent
TS28: main olfactory bulb	Absent
TS28: skeletal muscle	Absent
TS28: spinal cord	Absent
TS28: tongue	Absent

Structure	Level	Pattern	Image	Note
TS28: epididymis	Absent