Probe:	Six6 cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon floor plate	Weak	Regionally restricted		Faint expression was detected in the floor of the diencephalic portion of the prospective forebrain.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon floor plate	Present	Regionally restricted	2A , 2B	Expression was detected in the floor of the diencephalic portion of the prospective forebrain.
TS13: eye	Present	Regionally restricted	2B , 2A	Expression was detected in the presumptive ventral optic stalk.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong	Regionally restricted	2C	Strong expression was present in the presumptive pituitary/hypothalamic axis.
TS15: future neurohypophysis	Strong		2C	Strong expression was present in the presumptive pituitary/hypothalamic axis.
TS15: optic vesicle	Present	Regionally restricted	2C	Expression was detected in the rostral and caudal ventral portion of the optic vesicle.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Absent		2D

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	2F	Expression was limited to the ventral portion of the optic vesicle.
TS15: optic stalk	Present		2F

Structure	Level	Pattern	Image	Note
TS15: optic cup inner layer	Present	Regionally restricted	3A	Expression was detected in the ventral portion of the presumptive neural retina.
TS15: telencephalon	Absent		3A	Expression was absent in the ventral rostral forebrain.
TS15: olfactory placode	Present		3A

Structure	Level	Pattern	Image	Note
TS15: optic stalk	Present	Regionally restricted	3B	Expression was detected in the presumptive ventral optic stalk.
TS15: diencephalon	Present	Regionally restricted	3B	Expression was detected in the pituitary/hypothalamic axis.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present		4A
TS19: lens vesicle	Absent		4A

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present		4C
TS19: lens vesicle	Absent		4C

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present		4E
TS19: optic stalk	Present		4E
TS19: lens vesicle	Absent		4E
TS19: pigmented retinal epithelium	Absent		4E

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present		4G
TS19: lens vesicle	Absent		4G
TS19: pigmented retinal epithelium	Absent		4G

Structure	Level	Pattern	Image	Note
TS17: diencephalon lateral wall	Present	Regionally restricted	6A	Expression was detected in the hypothalamus.
TS17: infundibular recess of 3rd ventricle	Present		6A
TS17: Rathke's pouch	Present	Regionally restricted	6A	Expression was detected in the most dorsal part of Rathke's pouch lumen ectoderm.

Structure	Level	Pattern	Image	Note
TS17: diencephalon lateral wall	Present	Regionally restricted	6B	Expression was detected in the hypothalamus.
TS17: infundibular recess of 3rd ventricle	Present		6B
TS17: Rathke's pouch	Present	Regionally restricted	6B	Expression was detected in the most dorsal part of Rathke's pouch lumen ectoderm.

Structure	Level	Pattern	Image	Note
TS17: diencephalon lateral wall	Present	Regionally restricted	6C	Expression was detected in the hypothalamus.
TS17: infundibular recess of 3rd ventricle	Present		6C
TS17: Rathke's pouch	Present	Regionally restricted	6C	Expression was detected in the most dorsal part of Rathke's pouch lumen ectoderm.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall	Present	Regionally restricted	6D	Expression was detected in the hypothalamus.
TS19: infundibular recess of 3rd ventricle	Present		6D
TS19: Rathke's pouch	Present	Regionally restricted	6D	Expression was detected in the most dorsal part of Rathke's pouch lumen ectoderm.
TS19: nasal pit	Present	Regionally restricted	6D	Expression was detected in the olfactory placode.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	6E	Expression was detected in the hypothalamus.
TS19: telencephalon	Absent		6E

Structure	Level	Pattern	Image	Note
TS15: optic stalk	Present	Regionally restricted	8A wt	Expression was detected in the ventral optic stalk.
TS15: optic cup	Present	Regionally restricted	8A wt	Expression was detected in the ventral portion of the presumptive retina.

Structure	Level	Pattern	Image	Note
TS15: optic stalk	Present	Regionally restricted	8A Pax6ko	Expression was detected in the ventral optic stalk of the mutant embryos as in wild type.
TS15: optic cup	Present	Regionally restricted	8A Pax6ko	Expression was detected in the ventral portion of the presumptive retina of the mutant embryos as in wild type.

Structure	Level	Pattern	Image	Note
TS15: optic stalk	Present	Regionally restricted		Expression was detected in the ventral optic stalk.
TS15: optic cup	Present	Regionally restricted		Expression was detected in the ventral portion of the presumptive retina.

Structure	Level	Pattern	Image	Note
TS15: optic stalk	Present	Regionally restricted		Expression was detected in the ventral optic stalk of the mutant embryos as in wild type.
TS15: optic cup	Present	Regionally restricted		Expression was detected in the ventral portion of the presumptive retina of the mutant embryos as in wild type.