Probe:	Raldh2 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: branchial arch mesenchyme	Absent
TS15: fronto-nasal process mesenchyme	Absent
TS15: nervous system	Absent
TS15: optic vesicle	Absent
TS15: otocyst	Absent

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Regionally restricted		Expression is along each side of the maxillo-mandibular cleft and in the zone between first and second pharyngeal arches.
TS17: 2nd branchial arch	Present	Regionally restricted		Expression is along each side of the maxillo-mandibular cleft and in the zone between first and second pharyngeal arches.
TS17: somite	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Strong	Regionally restricted	1A	Expression is in the posterior half of the embryo, and in the mesoderm lateral to the primitive streak.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	1B	Expression is in the posterior half of the embryo, and in the mesoderm lateral to the primitive streak.
TS11: primitive streak	Weak		1B

Structure	Level	Pattern	Image	Note
TS12: embryo mesenchyme	Present	Regionally restricted	1C	Expression is in the entire posterior part of the embryo up to the base of the headfolds and surrounding the node.
TS12: allantois	Absent		1C
TS12: extraembryonic mesenchyme	Absent		1C
TS12: node	Absent		1C

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	1D	Expression extends rostrally along each side of the node.
TS11: node	Absent		1D

Structure	Level	Pattern	Image	Note
TS13: somite	Present	Regionally restricted	2A	Expression is in newly condensed somites and expression intensified in more mature (rostral) somites. Within the somite expression is strongest in the medial part.
TS13: allantois mesenchyme	Present	Regionally restricted	2A	Expression is in a ventral domain that surrounds the intraembryonic coelom at the level of the heart and in two symmetrical areas located ventrally to the hindgut diverticulum near the base of the allantois.
TS13: trunk mesenchyme	Present	Regionally restricted	2A	Expression is in a ventral domain that surrounds the intraembryonic coelom at the level of the heart and in two symmetrical areas located ventrally to the hindgut diverticulum near the base of the allantois.
TS13: embryo	Present	Regionally restricted	2A	Expression is in the posterior end.
TS13: head	Present	Regionally restricted	2A	Expression is in the eye region.

Structure	Level	Pattern	Image	Note
TS13: allantois	Present	Regionally restricted	2B	Expression is in ventral that surrounds the intraembryonic coelom at the level of the heart and in two symmetrical areas located ventrally to the hindgut diverticulum near the base of the allantois.
TS13: trunk mesenchyme	Present	Regionally restricted	2B	Expression is in ventral that surrounds the intraembryonic coelom at the level of the heart and in two symmetrical areas located ventrally to the hindgut diverticulum near the base of the allantois.

Structure	Level	Pattern	Image	Note
TS13: optic pit	Present		2C

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Strong	Regionally restricted	2D	Expression is in cervical mesenchyme and in mesenchyme surrounding the cervical spinal cord and spinal ganglia.
TS15: trunk somite	Present	Regionally restricted	2D	Expression is weak in the differentiated somites in the trunk region, but strong expression was seen in newer, more caudal somites.
TS15: trunk	Present	Regionally restricted	2D	Expression is in cloacal region towards the base of the allantois and in a lateral domain that extends from the cardiac region down to the cloacal domain, encompassing the base of the limb buds.
TS15: forelimb bud	Absent		2D
TS15: hindlimb ridge	Absent		2D

Structure	Level	Pattern	Image	Note
TS19: maxillary process	Strong		2E
TS19: future spinal cord	Present	Regionally restricted	2E	Expression is in the cervical and lumbar regions of the spinal cord.
TS19: 1st branchial arch	Present	Regionally restricted	2E	Expression is around the maxillo-mandibular cleft.
TS19: 2nd branchial arch	Present	Regionally restricted	2E	Expression is around the maxillo-mandibular cleft.
TS19: trigeminal ganglion	Present		2E
TS19: tail somite	Present		2E	Expression is in the most caudal somites in the cervical domain.
TS19: trunk somite	Absent		2E

Structure	Level	Pattern	Image	Note
TS19: handplate	Present	Regionally restricted	5A left	Expression is in a distal domain in the presumptive interdigital regions.

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region	Strong		5B left

Structure	Level	Pattern	Image	Note
TS22: forelimb interdigital region	Present	Regionally restricted	5C left

Structure	Level	Pattern	Image	Note
TS14: intraembryonic coelom	Present		8A
TS14: somite	Present		8A
TS14: trunk	Present	Regionally restricted	8A	Expression is in the cloacal region.

Structure	Level	Pattern	Image	Note
TS15: trunk	Strong	Regionally restricted	8B	Expression is in the cloacal region.