Robo1 RNA in situ Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:71421 Loes S, et al., Slit1 is specifically expressed in the primary and secondary enamel knots during molar tooth cusp formation. Mech Dev. 2001 Sep;107(1-2):155-7
Assay type:	RNA in situ
MGI Accession ID:	MGI:5424257
Gene symbol:	Robo1
Gene name:	roundabout guidance receptor 1

Probe:	Robo1 cDNA5
Probe preparation:	Antisense labelled with S35 RNA
Assay notes:	The exact developmental stages of the tooth germs were judged from the tissue sections according to morphological criteria.

Results

1D1 ©

Specimen 1D1: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: branchial arch mesenchyme	Present	Regionally restricted	1D1	Expression is in dental and jaw mesenchyme.

1D2 ©

Specimen 1D2: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: jaw primordium mesenchyme	Present		1D2
TS19: oral region	Present	Regionally restricted	1D2	Expression is in dental mesenchyme.

1D3 ©

Specimen 1D3: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: jaw primordium mesenchyme	Present		1D3
TS20: oral region	Present	Regionally restricted	1D3	Expression is in dental mesenchyme.

1D4 ©

Specimen 1D4: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: jaw mesenchyme	Present		1D4
TS21: molar mesenchyme	Present		1D4
TS21: primary enamel knot	Present		1D4
TS21: tooth bud	Present		1D4

1D5 ©

Specimen 1D5: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: molar mesenchyme	Present	Regionally restricted	1D5	Expression is in the middle of the tooth germ covering the enamel knot.
TS22: inner enamel epithelium	Present		1D5
TS22: molar dental papilla	Present		1D5
TS22: cap stage tooth	Present	Regionally restricted	1D5

1D6 ©

Specimen 1D6: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: molar dental papilla	Weak		1D6
TS24: inner enamel epithelium	Present	Regionally restricted	1D6	Expression is parts of the inner dental epithelium.
TS24: molar mesenchyme	Present	Regionally restricted	1D6	Expression is in parts next to the inner dental epithelium.
TS24: bell stage tooth	Present	Regionally restricted	1D6

1D7 ©

Specimen 1D7: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Weak		1D7
TS26: inner enamel epithelium	Present	Regionally restricted	1D7
TS26: molar mesenchyme	Present	Regionally restricted	1D7	Expression is in parts next to the inner dental epithelium.
TS26: bell stage tooth	Present	Regionally restricted	1D7

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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