Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Mir137tm1Mtm
Name:	microRNA 137; targeted mutation 1, Michael McManus
MGI ID:	MGI:5314770
Gene:	Mir137  Location: Chr3:118227506-118227578 bp, + strand  Genetic Position: Chr3, 51.71 cM
Alliance:	Mir137tm1Mtm page

Germline Transmission:	Earliest citation of germline transmission: J:181752
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin resistance gene cassette, a second FRT site and a second loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and a third loxP site immediately downstream of the microRNA stem loop. This was intended to create a knockout-first conditional-ready reporter knockout allele, but no beta-galactosidase activity could be detected in adult or embryonic tissues of heterozygous mice, nor could a functional conditional-ready allele be created after flp-mediated deletion of the insert, suggesting that the inserted FRT and/or LoxP recombination sites interfere with the transcription of the allele. (J:181752, J:244465)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Mir137 Mutation:	3 strains or lines available

Original:	J:181831 W.M. Keck Center for Noncoding RNAs, Information obtained from the W.M. Keck Center for Noncoding RNAs. Unpublished. 2012;
All:	3 reference(s)