Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 10, Liqun Luo |
MGI ID: |
MGI:5314256 |
Synonyms: |
R26TT |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo page
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Allele Type: |
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Targeted (Reporter) |
Mutation: |
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Insertion
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Mutation details: Cre-mediated recombination between Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo and Gt(ROSA)26Sortm7(ACTB-EGFP*)Luo produces this allele. The allele has the CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the recombined "MADM TT" cassette inserted into the Gt(ROSA)26Sor locus. The "MADM TT" cassette has an ATG start codon, a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] followed by a single loxP site), an ATG-less tdTomato sequence (nucleotides 3-1548 including stop codon) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal.
(J:181830)
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Generation of various Gt(ROSA)26Sor alleles |
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Original: |
J:181830 Tasic B, et al., Extensions of MADM (mosaic analysis with double markers) in mice. PLoS One. 2012;7(3):e33332 |
All: |
1 reference(s) |
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