Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 6, Liqun Luo
MGI ID:	MGI:5314252
Synonyms:	R26GT
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo page

Germline Transmission:	Earliest citation of germline transmission: J:181830
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Conditional ready, No functional change) Mutation:    Insertion   Mutation details: A targeting vector was created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "MADM GT" cassette. The "MADM GT" cassette used here (GFPN-terminus-intron-tdT3MycATG-less) was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP first exon; nucleotide 1-274), a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] and then a loxP-flanked neomycin resistance gene), an ATG-less tdTomato sequence (nucleotides 3-1548 including stop codon) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire construct was subcloned into the pROSA26-PA targeting vector to generate the final targeting construct pROSA26-GT. The pROSA26-GT construct was inserted into the Gt(ROSA)26Sor locus. (J:181830)

Generation of various Gt(ROSA)26Sor alleles

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:181830 Tasic B, et al., Extensions of MADM (mosaic analysis with double markers) in mice. PLoS One. 2012;7(3):e33332
All:	4 reference(s)