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Gene Expression Data
Assay Details
Assay
Reference: J:174887 Conover CA, et al., Pregnancy-associated plasma protein-A2 (PAPP-A2): tissue expression and biological consequences of gene knockout in mice. Endocrinology. 2011 Jul;152(7):2837-44
Assay type: RT-PCR
MGI Accession ID: MGI:5302810
Gene symbol: Pappa
Gene name: pregnancy-associated plasma protein A
Probe: Pappa-pC, Pappa-pD
Probe preparation: labelled with SYBR green
Assay notes: This is a quantitative RT-PCR and discussed in a table. Ribosomal protein L19 was used as the standard. Samples with copy numbers below 200 in absolute expression levels, corresponding to CT values of approximately 32, were considered to be the limit ofdetectable mRNA.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
Placenta E18.5 TS28: placenta Present 1 µg; total RNA Not Specified Female
Fetal head E18.5 TS26: head Present 1 µg; total RNA Not Specified Not Specified
Fetal body E18.5 TS26: trunk Present 1 µg; total RNA Not Specified Not Specified
Calvaria P w 8 TS28: chondrocranium Present (a) 1 µg; total RNA Not Specified Not Specified
Prostate P w 8 TS28: prostate gland Present 1 µg; total RNA Not Specified Not Specified
Colon P w 8 TS28: colon Present 1 µg; total RNA Not Specified Not Specified
Lung P w 8 TS28: lung Present 1 µg; total RNA Not Specified Not Specified
Ovaries P w 8 TS28: ovary Present (a) 1 µg; total RNA Not Specified Female
Tibia P w 8 TS28: tibia Present (a) 1 µg; total RNA Not Specified Not Specified
Brain P w 8 TS28: brain Present 1 µg; total RNA Not Specified Not Specified
Spinal cord P w 8 TS28: spinal cord Present (a) 1 µg; total RNA Not Specified Not Specified
Testes P w 8 TS28: testis Present (a) 1 µg; total RNA Not Specified Male
Kidney P w 8 TS28: metanephros Present (a) 1 µg; total RNA Not Specified Not Specified
Spleen P w 8 TS28: spleen Present 1 µg; total RNA Not Specified Not Specified
Soleus P w 8 TS28: soleus Present 1 µg; total RNA Not Specified Not Specified
Mesenteric adipose tissue P w 8 TS28: adipose tissue Present (a) 1 µg; total RNA Not Specified Not Specified
Thymus P w 8 TS28: thymus Present 1 µg; total RNA Not Specified Not Specified
Uterus P w 8 TS28: uterus Present (a) 1 µg; total RNA Not Specified Female
Lymph nodes P w 8 TS28: lymph node Present 1 µg; total RNA Not Specified Not Specified
Liver P w 8 TS28: liver Present 1 µg; total RNA Not Specified Not Specified
Subcutaneous adipose tissue P w 8 TS28: subcutaneous adipose tissue Present 1 µg; total RNA Not Specified Not Specified
Skin P w 8 TS28: skin Absent 1 µg; total RNA Not Specified Not Specified
Quadriceps muscle P w 8 TS28: upper leg muscle Present 1 µg; total RNA Not Specified Not Specified
Heart P w 8 TS28: heart Present (a) 1 µg; total RNA Not Specified Not Specified
Notes:
(a) Highest expression among all tested.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory