Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80025
Wallis D, et al., The zinc finger transcription factor Gfi1, implicated in lymphomagenesis, is required for inner ear hair cell differentiation and survival. Development. 2003 Jan;130(1):221-32
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5302554
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Gene symbol:
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Pou4f3
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Gene name:
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POU domain, class 4, transcription factor 3
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Probe:
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Brn3c probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3I:
embryonic day 18.5
(more  )
Specimen
3I:
(close  )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: organ of Corti |
Present |
Regionally restricted |
3I
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Expression is present in the hair cells.
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Specimen
3J:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Gfi1tm1Sho/Gfi1tm1Sho
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: organ of Corti |
Present |
Regionally restricted |
3J
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Expression is present in the hair cells; they are disorganized in the mutant and their numbers appear to be reduced.
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Analysis Tools