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Gene Expression Data
Assay Details
Assay
Reference: J:80020 Marcil A, et al., Pitx1 and Pitx2 are required for development of hindlimb buds. Development. 2003 Jan;130(1):45-55
Assay type: RNA in situ
MGI Accession ID: MGI:5298012
Gene symbol: Hand2
Gene name: heart and neural crest derivatives expressed 2
Probe: Hand2 probe2
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 5E top left: embryonic day 11.5 (more )
Note: This specimen is at the 47-somite stage.
Structure Level Pattern Image Note
TS19: hindlimb bud mesenchyme Present Regionally restricted 5E top left Expression is present in the posterior limb bud mesenchyme.
Specimen 5E bottom left: embryonic day 11.5; Pitx1tm1Jdr/Pitx1tm1Jdr (more )
Note: This specimen is at the 47-somite stage.
Structure Level Pattern Image Note
TS19: hindlimb bud mesenchyme Present Regionally restricted 5E bottom left Expression is extended anteriorly in the right limb compared to expression seen in wild type; expression in the left limb is more confined to the posterior mesenchyme, as in wild type.
Specimen 5E top middle: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 5E top middle Expression is present in the posterior limb bud mesenchyme.
Specimen 5E top right: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 5E top right Expression is present in the posterior limb bud mesenchyme.
Specimen 5E bottom middle: embryonic day 12.5; Pitx1tm1Jdr/Pitx1tm1Jdr, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 5E bottom middle Expression in the left limb is more confined to the posterior mesenchyme, as in wild type.
Specimen 5E bottom right: embryonic day 12.5; Pitx1tm1Jdr/Pitx1tm1Jdr, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 5E bottom right Expression is extended anteriorly in the right limb compared to expression seen in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory