Probe:	Dll1 probe18
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The probe is also possible being labelled with fluorescein, and detected by alkaline phosphatase-conjugated anti-fluorescein antibody.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	8B	Expression extends mediolaterally along its dorsoventral extent discontinuously, with two gaps adjacent to the lumen.
TS20: spinal cord floor plate	Absent		8B
TS20: spinal cord roof plate	Absent		8B

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	8C	Expression within the ventricular zone medial to Dll3 expression. Overlap of expression normally corresponds with the expression of Dll3 extending towards the lumen to the Dll1 domain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain ventricular layer	Present	Regionally restricted	8D	Expression in the ventricular zone adjacent to the lumen in domains spreading medial to lateral, coincide or separate from Dll3 expression. There are gaps adjacent to the lumen where Dll1 is not expressed.

Structure	Level	Pattern	Image	Note
TS20: hindbrain ventricular layer	Present	Regionally restricted	8E	Expression in the ventricular zone adjacent to the lumen in domains spreading medial to lateral, coincide or separate from Dll3 expression. There are gaps adjacent to the lumen where Dll1 is not expressed.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	8F	Express in patches in the lateral midbrain. Colocalise with Dll3 in the dorsal midbrain.
TS13: future prosencephalon	Present	Regionally restricted	8F	Express in patches in the future forebrain.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		8G right	Expression in much of the forebrain.

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present	Non-Uniform	8H	Posterior to the anterior Dll3-expression-only region, Dll1 and Dll3 are coexpressed with different relative levels creating 4 domains.

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present		8I	Colocalise within the presomitic mesoderm with Dll3 expression.
TS13: trunk somite	Present	Regionally restricted	8I	Expression in the posterior somite boundaries.

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Present	Regionally restricted	8J bottom	Expressed in a broad band presumably at the posterior of the forming somite. This domain narrows to the posterior boundary of the somites.
TS14: trunk unsegmented mesenchyme	Present		8J bottom	Colocalise within the presomitic mesoderm with Dll3 expression

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present