Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:142567
Capellini TD, et al., Conservation of notochord gene expression across chordates: insights from the Leprecan gene family. Genesis. 2008 Nov;46(11):683-96
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5295412
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Gene symbol:
|
P3h2
|
Gene name:
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prolyl 3-hydroxylase 2
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Probe:
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Leprel1 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
|
Alkaline phosphatase
|
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Results
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Specimen
4a:
embryonic day 9.5
(more )
Specimen
4a:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4b:
embryonic day 9.5
(more )
Specimen
4b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4d:
embryonic day 10.5
(more )
Specimen
4d:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4e/f:
embryonic day 10.5
(more )
Specimen
4e/f:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4g:
embryonic day 10.5
(more )
Specimen
4g:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4i:
embryonic day 11.5
(more )
Specimen
4i:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4j/k:
embryonic day 11.5
(more )
Specimen
4j/k:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: notochord |
Present |
Widespread |
4j
,
4k
|
Expression is present at all axial levels.
|
Specimen
4l/m:
embryonic day 11.5
(more )
Specimen
4l/m:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: notochord |
Present |
Widespread |
4m
,
4l
|
Expression is present at all axial levels.
|
Specimen
6a' E10.5:
embryonic day 10.5
(more )
Specimen
6a' E10.5:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6a' E11.5:
embryonic day 11.5
(more )
Specimen
6a' E11.5:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6a' E12.5:
embryonic day 12.5
(more )
Specimen
6a' E12.5:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6a' E13.5:
embryonic day 13.5
(more )
Specimen
6a' E13.5:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6b'h:
embryonic day 12.5
(more )
Specimen
6b'h:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6b'i:
embryonic day 12.5
(more )
Specimen
6b'i:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6b'j:
embryonic day 12.5
(more )
Specimen
6b'j:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6b'k:
embryonic day 13.5
(more )
Specimen
6b'k:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb |
Weak |
|
6b'k
|
Expression is present in the proximal limb.
|
Specimen
6b'l:
embryonic day 13.5
(more )
Specimen
6b'l:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6b'm:
embryonic day 13.5
(more )
Specimen
6b'm:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|