Antibody:	mMR antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Fixative was either 10% formaldehyde or 10% formaldehyde mixed with 50% benzoquinone and 5% acetic acid.

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present	Regionally restricted	1B	Expression is on a vessel wall of blood islands in the mesothelium.

Structure	Level	Pattern	Image	Note
TS17: yolk sac	Present	Single cells	1D	Staining is extended along the elongated vessel wall.

Structure	Level	Pattern	Image	Note
TS21: yolk sac visceral endoderm	Present	Regionally restricted	1F	Expression is on the vessel wall and in macrophages in the vessel lumen.

Structure	Level	Pattern	Image	Note
TS25: bone marrow	Present	Single cells	2B	Expression is on a parenchymal macrophage cell and a sinusoidal lining cell.

Structure	Level	Pattern	Image	Note
TS27: bone marrow	Strong	Regionally restricted	2D	Expression is on sinusoidal endothelial cells an on single cells in the bone marrow stroma.

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present	Regionally restricted	2F	Expression is on sinusoidal endothelial cells. It is also present in a subset of macrophages in the bone marrow.

Structure	Level	Pattern	Image	Note
TS19: liver parenchyma	Present	Single cells	3B	Expression is on round macrophages.
TS19: liver sinusoid	Present	Single cells	3B	Expression is localized on the thin wall of the sinusoidal lumen (endothelial cells).

Structure	Level	Pattern	Image	Note
TS21: liver sinusoid	Present	Regionally restricted	3D	Expression is localized on the thin wall of the sinusoidal lumen (endothelial cells).
TS21: liver parenchyma	Present	Single cells	3D	Expression is on round macrophages.

Structure	Level	Pattern	Image	Note
TS25: liver	Present	Regionally restricted	3F	Expression is on round macrophages. Expression is localized on the thin wall of the sinusoidal lumen (endothelial cells).

Structure	Level	Pattern	Image	Note
TS27: liver sinusoid	Present	Regionally restricted	3H	Staining is on sinusoidal endothelial cells.
TS27: liver parenchyma	Present	Single cells	3H	Staining is on Kupffer cells.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Single cells	3J	Expression is on Kupffer cells.
TS28: liver sinusoid	Present	Regionally restricted	3J	Staining is on sinusoidal endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: liver sinusoid	Present		3L
TS28: liver	Present	Single cells	3L	Expression is on Kupffer cells.

Structure	Level	Pattern	Image	Note
TS27: lymph node medulla	Present	Regionally restricted	5B	Macrophages in the lymph node and in sinusoidal endothelial cells.
TS27: lymph node cortex	Present	Regionally restricted	5B	Expression is beneath the capsule.

Structure	Level	Pattern	Image	Note
TS28: lymph node medulla	Present	Regionally restricted	5D	Macrophages in the lymph node and on endothelial sinusoidal cells.
TS28: lymph node cortex	Present	Regionally restricted	5D	Expression is beneath the capsule.

Structure	Level	Pattern	Image	Note
TS28: lymph node medulla	Present	Regionally restricted	5F	Macrophages in the lymph node and on sinusoidal endothelial cells.
TS28: lymph node cortex	Present	Regionally restricted	5F	Expression is beneath the capsule.

Structure	Level	Pattern	Image	Note
TS23: intestine	Present	Single cells	6B	Expression is on macrophages.

Structure	Level	Pattern	Image	Note
TS27: intestinal villus	Present	Regionally restricted	6D	Expression is in the central area.
TS27: small intestine lamina propria	Present	Single cells	6D	Macrophages in the lamina propria.

Structure	Level	Pattern	Image	Note
TS28: intestinal villus	Present	Regionally restricted	6F	Expression is localized to the thin walls of lymphatic channels along with lymphatic endothelial cells.
TS28: lymphatic vessel	Present	Regionally restricted	6F	Expression is localized to the thin walls of lymphatic channels along with lymphatic endothelial cells.

Structure	Level	Pattern	Image	Note
TS22: spleen primordium	Present	Scattered	7B

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Trace		7D
TS28: spleen white pulp	Absent		7D	Absent in marginal zone and splenic endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Present		7F
TS28: spleen white pulp	Trace		7F

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Present	Single cells	7H	Expression present in macrophages.
TS28: spleen white pulp	Trace	Single cells	7H

Structure	Level	Pattern	Image	Note
TS21: thymus primordium	Present	Regionally restricted	8B	Expression is in flattened cells beneath the mesenchyme and in scattered cells in the epithelium.

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Present	Regionally restricted	8D	Expression is beneath the capsule in flattened cells and in scattered cells in the epithelium.

Structure	Level	Pattern	Image	Note
TS28: thymus cortex	Present		8F
TS28: thymus medullary core	Present		8F

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Single cells	9B	Positive cells are scattered throughout the mesenchyme on interstitial macrophages, absent on endothelial cells.

Structure	Level	Pattern	Image	Note
TS26: lung alveolus	Present	Single cells	9D	Expression is in alveolar macrophages absent on endothelial cells.

Structure	Level	Pattern	Image	Note
TS27: lung alveolus	Present		9F	Expression is in alveolar macrophages absent on endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: lung alveolus	Present		9H	Expression is in alveolar macrophages absent on endothelial cells.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present	Single cells	10B	Expression is on macrophages.
TS21: epidermis	Present	Single cells	10B	Expression is on Langerhans cells.

Structure	Level	Pattern	Image	Note
TS24: dermis	Present	Single cells	10D	Expression is on macrophages.
TS24: epidermis	Present	Single cells	10D	Expression is on Langerhans cells.

Structure	Level	Pattern	Image	Note
TS28: dermis	Present	Single cells	10F	Expression is on macrophages.
TS28: epidermis	Present	Single cells	10F	Expression is on Langerhans cells.

Structure	Level	Pattern	Image	Note
TS28: epidermis	Present	Single cells	10G	Expression is on Langerhans cells.