Gene Expression Data
Assay Details
Assay
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Reference:
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J:162481
Hoesel B, et al., Combination of in silico and insitu hybridisation approaches to identify potential Dll1 associated miRNAs during mouse embryogenesis. Gene Expr Patterns. 2010 Sep;10(6):265-73
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5287490
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Gene symbol:
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Mir130b
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Gene name:
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microRNA 130b
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Probe:
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miR-130b probe
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Probe preparation:
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Antisense labelled with digoxigenin DNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The double-DIG labeled probe has label at both the 3' and 5' ends.
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Results
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Specimen
2P:
embryonic day 9.5
(more )
Note: A single-DIG labeled (SL) probe was used on this specimen.
Specimen
2P:
(close )
Genetic Background:
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C3HeB/FeJ
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Age:
|
embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
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A single-DIG labeled (SL) probe was used on this specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: somite |
Present |
Widespread |
2P
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Expression is detected in all somites along the posterior anterior axis.
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Specimen
2R:
embryonic day 9.5
(more )
Note: A double-DIG labeled (DL) probe was used on this specimen.
Specimen
2R:
(close )
Genetic Background:
|
C3HeB/FeJ
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Age:
|
embryonic day 9.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
A double-DIG labeled (DL) probe was used on this specimen.
|
Specimen
3K:
embryonic day 10.5
(more )
Note: A double-DIG labeled (DL) probe was used on this specimen.
Specimen
3K:
(close )
Genetic Background:
|
C3HeB/FeJ
|
Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
A double-DIG labeled (DL) probe was used on this specimen.
|
Specimen
3L-first:
embryonic day 10.5
(more )
Specimen
3L-first:
(close )
Genetic Background:
|
C3HeB/FeJ
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Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
3L-second:
embryonic day 10.5
(more )
Specimen
3L-second:
(close )
Genetic Background:
|
C3HeB/FeJ
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Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
3L-third:
embryonic day 10.5
(more )
Specimen
3L-third:
(close )
Genetic Background:
|
C3HeB/FeJ
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Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3L-fourth:
embryonic day 10.5
(more )
Specimen
3L-fourth:
(close )
Genetic Background:
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C3HeB/FeJ
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Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS17: somite |
Present |
Non-Uniform |
3L-fourth
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In older, more anterior, somites, expression is strongest in the posterior compartment of each somite.
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Specimen
3L-fifth:
embryonic day 10.5
(more )
Note: A single-DIG labeled (SL) probe was used on this specimen.
Specimen
3L-fifth:
(close )
Genetic Background:
|
C3HeB/FeJ
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Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
A single-DIG labeled (SL) probe was used on this specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: somite |
Present |
Non-Uniform |
3L-fifth
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In the more recently formed, most posterior somites, expression is present in the whole somite; it is detected at the inner epithelial walls without restriction to the posterior compartments.
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Specimen
4C:
embryonic day 9.5
(more )
Note: A single-DIG labeled (SL) probe was used on this specimen.
Specimen
4C:
(close )
Genetic Background:
|
C3HeB/FeJ
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
A single-DIG labeled (SL) probe was used on this specimen.
|
Specimen
Not shown:
embryonic day 9.5
(more )
Note: A double-DIG labeled (DL) probe was used on this specimen.
Specimen
Not shown:
(close )
Genetic Background:
|
C3HeB/FeJ
|
Age:
|
embryonic day 9.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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A double-DIG labeled (DL) probe was used on this specimen.
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Specimen
4H:
embryonic day 10.5
(more )
Note: A single-DIG labeled (SL) probe was used on this specimen.
Specimen
4H:
(close )
Genetic Background:
|
C3HeB/FeJ
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Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
A single-DIG labeled (SL) probe was used on this specimen.
|
Specimen
Not shown:
embryonic day 10.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
C3HeB/FeJ
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5F:
embryonic day 10.5
(more )
Note: A single-DIG labeled (SL) probe was used on this specimen. This section is through the trunk region containg the posterior 4-5 most recently formed somites.
Specimen
5F:
(close )
Genetic Background:
|
C3HeB/FeJ
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Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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A single-DIG labeled (SL) probe was used on this specimen. This section is through the trunk region containg the posterior 4-5 most recently formed somites.
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Specimen
5G:
embryonic day 10.5
(more )
Note: This section is through the trunk region containg the posterior 4-5 most recently formed somites.
Specimen
5G:
(close )
Genetic Background:
|
C3HeB/FeJ
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Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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This section is through the trunk region containg the posterior 4-5 most recently formed somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk somite |
Present |
Regionally restricted |
5G
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Expression is present in the second and third most posterior somite.
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