Gene Expression Data
Assay Details
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Assay
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Reference:
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J:142123
Ishimura A, et al., Man1, an inner nuclear membrane protein, regulates left-right axis formation by controlling nodal signaling in a node-independent manner. Dev Dyn. 2008 Dec;237(12):3565-76
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5287246
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Probe:
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Nodal probe12
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
Table 1 1s wild type:
embryonic day 8.0
(more  )
Note: 1 somite stage.
Specimen
Table 1 1s wild type:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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1 somite stage.
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Specimen
Table 1 1s Man1 mutant:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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1 somite stage.
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Specimen
2A:
embryonic day 8.0
(more )
Note: 2 somite stage.
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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2 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2A
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Expression was detected in the left lateral plate mesoderm.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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2 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2B
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Ectopic bilateral expression was detected in the anterior lateral plate mesoderm in addition to the normal expression domain in the posterior left lateral plate mesoderm.
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Specimen
2C:
embryonic day 8.0
(more )
Note: 4-6 somite stage.
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2C
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Expression was detected in the left lateral plate mesoderm. By the 4 somite stage the expression domain had expanded anteriorly and posteriorly compared to the 2 somite stage.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2D
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Expression was elevated markedly in both sides of the lateral plate mesoderm.
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Specimen
2E:
embryonic day 8.0
(more )
Note: 4-6 somite stage.
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2E
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Expression was detected in the left lateral plate mesoderm. By the 4 somite stage the expression domain had expanded anteriorly and posteriorly compared to the 2 somite stage.
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2F
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Expression was elevated markedly in both sides of the lateral plate mesoderm. In 6 out of 10 embryos ectopic expression in the right had expanded posteriorly although it was weaker in intensity compared to the left.
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Specimen
2G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2G
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Expression was elevated markedly in both sides of the lateral plate mesoderm. In two thirds of the embryos ectopic expression in the right posterior region showed discrete anterior and posterior expression domains.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2H
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Expression was elevated markedly in both sides of the lateral plate mesoderm. In two thirds of the embryos ectopic expression in the right posterior region showed discrete anterior and posterior expression domains.
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Specimen
2I:
embryonic day 8.5
(more )
Note: 8-10 somite stage.
Specimen
2I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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8-10 somite stage.
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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8-10 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
2J
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Bilateral expression in the lateral plate mesoderm continued even to 7-9 somite stage when expression normally turns off.
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Specimen
6A:
embryonic day 8.0
(more )
Note: 4 somite stage.
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
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6A
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Inset shows the node region in higher magnification.
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| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
6A
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Expression was detected in the left lateral plate mesoderm.
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Specimen
6B:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6N) or (involves: 129S1/Sv * 129T2/SvEms * 129X1/SvJ)
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Mutant Allele(s):
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Nodaltm1Hmd/Nodaltm1Hmd
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somite stage.
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
Nodaltm1Hmd/Nodaltm1Hmd
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Absent |
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6C
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Inset shows the node region in higher magnification.
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| TS12: lateral plate mesoderm |
Strong |
Regionally restricted |
6C
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Strong, bilateral expression was detected in the lateral plate mesoderm.
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Analysis Tools