Antibody:	KSR antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	For sectioning, embryos and tissues were either embedded in Tissue-Tek OCT compound (Sakura) in cryogenic molds and solidified on a dry ice-hexane bath or fixed in Methacarn (10% (v/v) glacial acetic acid, 30% (v/v) chloroform, 60% (v/v) methanol), and paraffin embedded.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Very strong	Regionally restricted	3A	Expression is in the cell bodies of the pyramidal neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Very strong		3B

Structure	Level	Pattern	Image	Note
TS28: ovary stratum granulosum	Present		3C

Structure	Level	Pattern	Image	Note
TS28: alveolus epithelium	Present		3D	Localization is primarily at the plasma membrane region.

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Strong		3E	Localization is primarily at the plasma membrane region.
TS28: secondary spermatocyte	Strong		3E	Localization is primarily at the plasma membrane region.

Structure	Level	Pattern	Image	Note
TS28: epithelial layer of infundibulum	Present

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		4A
TS9: embryo endoderm	Present		4A
TS9: embryo mesoderm	Present		4A
TS9: extraembryonic component	Present	Regionally restricted	4A	Expression is in the cell layers of the amnion and the extraembryonic membranes.

Structure	Level	Pattern	Image	Note
TS17: brain	Strong		4B
TS17: heart	Strong		4B
TS17: limb	Strong		4B
TS17: tail	Strong		4B

Structure	Level	Pattern	Image	Note
TS17: brain	Strong		4C
TS17: heart	Strong		4C
TS17: limb	Strong		4C
TS17: tail	Strong		4C