Probe:	EEN-B2 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: branchial arch ectoderm	Present		4I
TS17: dorsal aorta	Present		4I
TS17: future spinal cord	Present		4I
TS17: midbrain	Present		4I
TS17: telencephalon	Present		4I
TS17: hindbrain	Present	Regionally restricted	4I	Expression is in the myelencephalon.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Uniform	4N	Expression is in almost all neurons in the spinal cord and brain.
TS20: spinal cord	Present	Uniform	4N	Expression is in almost all neurons in the spinal cord and brain.
TS20: peripheral nervous system	Present		4N
TS20: cortical plate	Strong	Regionally restricted	4N	Expression is in the outermost layer of the cortical plate.
TS20: spinal ganglion	Strong		4N
TS20: intestine	Weak		4N
TS20: lung	Weak		4N
TS20: metanephros	Weak		4N

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Strong		4S
TS22: main olfactory bulb	Strong		4S
TS22: adrenal gland	Present		4S
TS22: cochlea	Present		4S
TS22: cranial ganglion	Present		4S
TS22: intestine	Present		4S
TS22: lung	Present		4S
TS22: metanephros	Present		4S

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		5D
TS28: cerebral cortex	Strong		5D
TS28: dentate gyrus	Strong		5D
TS28: hippocampus pyramidal cell layer	Strong		5D
TS28: brainstem	Present		5D
TS28: corpus striatum	Present		5D
TS28: hippocampus	Present		5D
TS28: thalamus	Present		5D

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Strong	Regionally restricted	5H	Expression is in the pyramidal cells.
TS28: hippocampus CA1	Present	Regionally restricted	5H	Expression is in the pyramidal cells.
TS28: hippocampus CA2	Present	Regionally restricted	5H	Expression is in the pyramidal cells.
TS28: hippocampus CA4	Present	Regionally restricted	5H	Expression is in the pyramidal cells.
TS28: dentate gyrus	Present		5H

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		5L
TS28: cortical layer I	Absent		5L

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5P
TS28: cerebellum granule cell layer	Present		5P
TS28: cerebellum molecular layer	Weak		5P

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong	Single cells	5T	Expression is in cells that have morphological characteristics consistent with neurons and is absent in glial cells.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Strong		5X

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule epithelium	Present	Regionally restricted	5CC	Expression is in the basal lamina of the seminiferous epithelium (Sertoli cells and immature spermatogonia).
TS28: spermatogonium	Present		5CC