Probe:	EEN-B1 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: branchial arch ectoderm	Present		4H
TS17: dorsal aorta	Present		4H
TS17: future spinal cord	Present		4H
TS17: midbrain	Present		4H
TS17: telencephalon	Present		4H
TS17: hindbrain	Present	Regionally restricted	4H	Expression is in the myelencephalon.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Uniform	4M	Expression is in almost all neurons in the spinal cord and brain.
TS20: spinal cord	Present	Uniform	4M	Expression is in almost all neurons in the spinal cord and brain.
TS20: peripheral nervous system	Present		4M
TS20: cortical plate	Strong	Regionally restricted	4M	Expression is in the outermost layer of the cortical plate.
TS20: spinal ganglion	Strong		4M
TS20: intestine	Weak		4M
TS20: lung	Weak		4M
TS20: metanephros	Weak		4M

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Strong		4R
TS22: main olfactory bulb	Strong		4R
TS22: adrenal gland	Present		4R
TS22: cochlea	Present		4R
TS22: cranial ganglion	Present		4R
TS22: intestine	Present		4R
TS22: lung	Present		4R
TS22: metanephros	Present		4R

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		5C
TS28: cerebral cortex	Strong		5C
TS28: dentate gyrus	Strong		5C
TS28: hippocampus pyramidal cell layer	Strong		5C
TS28: brainstem	Strong		5C
TS28: corpus striatum	Strong		5C
TS28: hippocampus	Strong		5C
TS28: thalamus	Strong		5C

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Strong	Regionally restricted	5G	Expression is in the pyramidal cells.
TS28: hippocampus CA1	Present	Regionally restricted	5G	Expression is in the pyramidal cells.
TS28: hippocampus CA2	Present	Regionally restricted	5G	Expression is in the pyramidal cells.
TS28: hippocampus CA4	Present	Regionally restricted	5G	Expression is in the pyramidal cells.
TS28: dentate gyrus	Present		5G

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		5K
TS28: cortical layer I	Absent		5K

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5O
TS28: cerebellum granule cell layer	Present		5O
TS28: cerebellum molecular layer	Weak		5O

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong	Single cells	5S	Expression is in cells that have morphological characteristics consistent with neurons and is absent in glial cells.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Strong		5W

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule epithelium	Present	Regionally restricted	5BB	Expression is in the basal lamina of the seminiferous epithelium (Sertoli cells and immature spermatogonia).
TS28: spermatogonium	Present		5BB