Antibody:	mDuffy antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3A

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	3B	Expression is in the outer differentiated zone near the periphery of the neural tube.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	3C	Expression is in the sensory tracts extending from the spinal ganglia into the neural tube.
TS20: spinal ganglion	Present	Regionally restricted	3C	Expression is in the sensory tracts extending from the spinal ganglia into the neural tube.

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Spotted	3D

Structure	Level	Pattern	Image	Note
TS21: enteric nervous system	Strong		4C
TS21: gut epithelium	Absent		4C
TS21: gut mesenchyme	Absent		4C

Structure	Level	Pattern	Image	Note
TS20: parasympathetic nervous system	Strong	Regionally restricted	5E	Expression is in the sympathetic ganglia in the noncellular regions.
TS20: blood vessel	Absent		5E

Structure	Level	Pattern	Image	Note
TS19: heart	Absent		6D

Structure	Level	Pattern	Image	Note
TS19: floor plate	Strong
TS19: neural tube	Present	Regionally restricted		Expression is in fibrous nerve tracts at the periphery of the differentiating neural tube and in axonal tracts.
TS19: blood vessel	Absent
TS19: limb	Absent

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted		Expression is in the olfactory bulb.
TS20: central nervous system nerve	Present	Regionally restricted		Expression is in the axonal fibres of the olfactory nerve.
TS20: hindbrain	Present	Regionally restricted		Expression is in motor neurons.

Structure	Level	Pattern	Image	Note
TS21: central nervous system nerve	Present			Expression is in the neuronal tracts and fibres.
TS21: spinal nerve	Present			Expression is in the neuronal tracts and fibres.
TS21: blood	Present	Single cells		Expression is in the liver.
TS21: blood vessel	Absent

Structure	Level	Pattern	Image	Note
TS22: dermis	Present	Regionally restricted		Expression is in the nerve tracts around the hair follicles.
TS22: inner ear mesenchyme	Present
TS22: lung mesenchyme	Present
TS22: blood	Present			Expression is in red blood cells in the lung.