Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:170493
Pfister S, et al., Sox17-dependent gene expression and early heart and gut development in Sox17-deficient mouse embryos. Int J Dev Biol. 2011;55(1):45-58
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:4949215
|
|
Gene symbol:
|
Myocd
|
|
Gene name:
|
myocardin
|
|
Probe:
|
Myocd cDNA2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
5B:
embryonic day 8.25
(more  )
Note: Pre-somite stage.
Specimen
5B:
(close  )
|
Genetic Background:
|
involves: 129 * 129S1/Sv
|
|
Age:
|
embryonic day 8.25
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Pre-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: heart |
Present |
|
5B
|
|
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129 * 129S1/Sv
|
|
Mutant Allele(s):
|
Sox17tm1Ysk/Sox17tm1Ysk
|
|
Age:
|
embryonic day 8.25
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Pre-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: heart |
Present |
Regionally restricted |
5C
|
Expression is absent in the midline tissues in the primary heart field.
|
Specimen
6A:
embryonic day 8.5
(more )
Note: 7-8 somites.
Specimen
6A:
(close )
|
Genetic Background:
|
involves: 129 * 129S1/Sv
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
7-8 somites.
|
Specimen
6B:
(close )
|
Genetic Background:
|
involves: 129 * 129S1/Sv
|
|
Mutant Allele(s):
|
Sox17tm1Ysk/Sox17tm1Ysk
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
7-8 somites.
|
Specimen
6C:
(close )
|
Genetic Background:
|
involves: 129 * 129S1/Sv
|
|
Mutant Allele(s):
|
Sox17tm1Ysk/Sox17tm1Ysk
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
7-8 somites.
|
Specimen
6D:
(close )
|
Genetic Background:
|
involves: 129 * 129S1/Sv
|
|
Mutant Allele(s):
|
Sox17tm1Ysk/Sox17tm1Ysk
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
7-8 somites.
|
Specimen
6E,I:
embryonic day 8.5
(more )
Note: 7-8 somites. Sections of embryo shown in Fig. 6A.
Specimen
6E,I:
(close )
|
Genetic Background:
|
involves: 129 * 129S1/Sv
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
7-8 somites. Sections of embryo shown in Fig. 6A.
|
Specimen
6F,J:
embryonic day 8.5; Sox17tm1Ysk/ Sox17tm1Ysk
(more )
Note: 7-8 somites. Sections of embryo shown in Fig. 6B.
Specimen
6F,J:
(close )
|
Genetic Background:
|
involves: 129 * 129S1/Sv
|
|
Mutant Allele(s):
|
Sox17tm1Ysk/Sox17tm1Ysk
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
7-8 somites. Sections of embryo shown in Fig. 6B.
|
Specimen
6G,K:
embryonic day 8.5; Sox17tm1Ysk/ Sox17tm1Ysk
(more )
Note: 7-8 somites. Sections of embryo shown in Fig. 6C.
Specimen
6G,K:
(close )
|
Genetic Background:
|
involves: 129 * 129S1/Sv
|
|
Mutant Allele(s):
|
Sox17tm1Ysk/Sox17tm1Ysk
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
7-8 somites. Sections of embryo shown in Fig. 6C.
|
Specimen
6H,L:
embryonic day 8.5; Sox17tm1Ysk/ Sox17tm1Ysk
(more )
Note: 7-8 somites. Sections of embryo shown in Fig. 6D.
Specimen
6H,L:
(close )
|
Genetic Background:
|
involves: 129 * 129S1/Sv
|
|
Mutant Allele(s):
|
Sox17tm1Ysk/Sox17tm1Ysk
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
7-8 somites. Sections of embryo shown in Fig. 6D.
|
|
Analysis Tools