Antibody:	anti-nAchRa5
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		1A	Expression is present in the primitive airway epithelium.
TS21: lung mesenchyme	Present	Scattered	1A	There is sporadic expression in pulmonary mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present		1B

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Present		1C

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		1D	Expression is present in primitive respiratory epithelium as well as larger airways. Expression is restricted to the luminal air surfaces.

Structure	Level	Pattern	Image	Note
TS27: lung epithelium	Present	Non-Uniform	1E	There is marked expression in the proximal lung airways and only minimal expression in the peripheral lung.

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Regionally restricted	1F	Expression is present in lung parenchyma and in the peripheral respiratory region. It is noticeably absent in the airways.

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Non-Uniform	1G	Expression is present in the respiratory compartment. There is minimal to no expression in the proximal lung.

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		1H	There is abundant expression in the lung, including in the proximal and distal pulmonary epithelium.

Structure	Level	Pattern	Image	Note
TS27: alveolus epithelium	Present	Regionally restricted	3B	Expression is present in many type II alveolar cells but not all.

Structure	Level	Pattern	Image	Note
TS27: bronchiole epithelium	Present		3D	Expression is present in many Clara cells.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Trace		7A	There is an almost complete ablation of expression in the mutant.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Strong		7B	Expression is present in proximal and distal pulmonary epithelium.