Probe:	Vangl1 cDNA1
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: embryo	Trace		1F

Structure	Level	Pattern	Image	Note
TS20: liver	Strong		2P
TS20: cranial ganglion	Moderate		2P
TS20: dorsal root ganglion	Moderate		2P
TS20: central nervous system	Weak		2P
TS20: brain	Present	Regionally restricted	2P	A small focus of expression was associated with the raphe of the brainstem.

Structure	Level	Pattern	Image	Note
TS22: embryo	Weak	Widespread		Expression was detected in almost all non-neural tissue, with highest intensity in the skin and liver.
TS22: liver	Present			Expression in the skin and liver was lower at E14.5 than at E12.5.
TS22: skin	Present			Expression in the skin and liver was lower at E14.5 than at E12.5.
TS22: brain	Present	Regionally restricted		No signal was seen in the brain, except in the raphe in the brainstem.
TS22: brainstem	Present	Regionally restricted		No signal was seen in the brain, except in the raphe in the brainstem.

Structure	Level	Pattern	Image	Note
TS25: brainstem	Present	Regionally restricted		Expression was present in the reticular formation and the cochleovestibular nuclei.
TS25: cerebellum	Present	Regionally restricted		Expression was present in the external granule layer.
TS25: diencephalon ventricular layer	Ambiguous			Expression was very low or undetectable.
TS25: hypothalamus	Ambiguous			Expression was very low or undetectable.
TS25: midbrain tegmentum	Ambiguous			Expression was very low or undetectable.
TS25: midbrain ventricular layer	Ambiguous			Expression was very low or undetectable.
TS25: tectum	Ambiguous			Expression was very low or undetectable.
TS25: thalamus	Present	Regionally restricted		Expression was detected in the dorsal thalamus but was very low or undetectable in the ventral thalamus.
TS25: retina	Ambiguous	Regionally restricted		Expression was very low or undetectable in the retina ventricular zone and ganglion cells.
TS25: cortical plate	Ambiguous	Regionally restricted		Expression was very low or undetectable in the ventricular zone/subventricular zone and the cortical plate proper.
TS25: corpus striatum	Ambiguous	Regionally restricted		Expression was very low or undetectable in the striatum ventricular zone/subventricular zone and the striatum.
TS25: main olfactory bulb	Ambiguous	Regionally restricted		Expression was very low or undetectable in the olfactory bulb and mitral cells.

Structure	Level	Pattern	Image	Note
TS25: hair bulb	Present		4F1
TS25: pituitary gland	Present		4F1
TS25: skin	Present		4F1
TS25: spinal cord ventricular layer	Strong		4F1
TS25: hindbrain ventricular layer	Strong	Regionally restricted	4F1	Expression was strong in the ventricular lining of the fourth ventricle and aqueduct.
TS25: midbrain ventricular layer	Strong	Regionally restricted	4F1	Expression was strong in the ventricular lining of the fourth ventricle and aqueduct.
TS25: epithalamus	Present	Regionally restricted	4F1	Expression was detected in the habenular nuclei.

Structure	Level	Pattern	Image	Note
TS27: brain	Trace		5I
TS27: habenula	Present	Regionally restricted	5I	Expression was detected in habenular nuclei.

Structure	Level	Pattern	Image	Note
TS27: midbrain	Present	Regionally restricted		Expression was detected in some nuclei in the basal mesencephalon.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Ambiguous			Expression was very low or undetectable.
TS28: diencephalon	Ambiguous	Regionally restricted		Expression was very low or undetectable in the diencephalon ventricular zone.
TS28: dentate gyrus	Ambiguous			Expression was very low or undetectable.
TS28: dorsal thalamus	Ambiguous			Expression was very low or undetectable.
TS28: hippocampus pyramidal cell layer	Ambiguous			Expression was very low or undetectable.
TS28: hypothalamus	Ambiguous			Expression was very low or undetectable.
TS28: main olfactory bulb granule cell layer	Ambiguous			Expression was very low or undetectable.
TS28: main olfactory bulb mitral cell layer	Ambiguous			Expression was very low or undetectable.
TS28: prethalamus	Ambiguous			Expression was very low or undetectable.
TS28: cerebral cortex	Ambiguous	Regionally restricted		Expression was very low or undetectable in the cortical plate proper and ventricular zone/subventricular zone.
TS28: corpus striatum	Ambiguous	Regionally restricted		Expression was very low or undetectable in the striatum and the ventricular zone/subventricular zone.
TS28: rostral migratory stream	Ambiguous	Regionally restricted		Expression was very low or undetectable in the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: brain	Absent