Gene Expression Data
Assay Details
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Assay
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Reference:
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J:90402
Merrill BJ, et al., Tcf3: a transcriptional regulator of axis induction in the early embryo. Development. 2004 Jan;131(2):263-74
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4943172
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Gene symbol:
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Egr2
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Gene name:
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early growth response 2
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Probe:
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Krox20 probe21
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4E:
embryonic day 8.5
(more  )
Note: Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
Specimen
4E:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
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Specimen
4E':
embryonic day 8.5
(more )
Note: Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
Specimen
4E':
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
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Specimen
Heterozygote:
embryonic day 8.5; Tcf7l1tm2Efu/Tcf7l1 +
(more )
Specimen
Heterozygote:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4F:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Weak |
Regionally restricted |
4F
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Expression is restricted to a single band on each neural fold and is markedly reduced in intensity compared to what is seen in wild type.
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Specimen
4F':
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Weak |
Regionally restricted |
4F'
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Expression is restricted to a single band on each neural fold and is markedly reduced in intensity compared to what is seen in wild type. Also, it is expanded to the lateral surfaces of the neural folds, rather than being confined to the grooves.
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Analysis Tools