Gene Expression Data
Assay Details
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Assay
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Reference:
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J:90402
Merrill BJ, et al., Tcf3: a transcriptional regulator of axis induction in the early embryo. Development. 2004 Jan;131(2):263-74
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4943171
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Probe:
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En1 probe11
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4C:
embryonic day 8.5
(more  )
Note: Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
Specimen
4C:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Regionally restricted |
4C
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Expression is present in the neurectoderm at the caudal end of the midbrain.
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Specimen
4C':
embryonic day 8.5
(more )
Note: Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
Specimen
4C':
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Regionally restricted |
4C'
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Expression is present in the neurectoderm at the caudal end of the midbrain.
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Specimen
Heterozygote:
embryonic day 8.5; Tcf7l1tm2Efu/Tcf7l1 +
(more )
Specimen
Heterozygote:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Regionally restricted |
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Expression is present in the neurectoderm at the caudal end of the midbrain.
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Specimen
4D:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
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4D
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In the mutant, expression is expanded rostrally and its distance from the rostral tip of the embryo is shortened, consistent with a severe reduction of the forebrain.
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Specimen
4D':
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
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4D'
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In the mutant, expression is expanded rostrally and its distance from the rostral tip of the embryo is shortened, consistent with a severe reduction of the forebrain.
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Analysis Tools